Anti-Phosphotyrosine Antibody, clone PY20, HRP Conjugate

半岛bd体育手机客户端 说明

一般描述

Protein tyrosine phosphorylation was first described in 1979. Large numbers of tyrosine kinases have been described since, and many of the signaling pathways initiated by tyrosine phosphorylation have been delineated. Availability of a top-quality anti-phosphotyrosine antibody has allowed the study of tyrosine kinase activity by simple blotting procedures, and without routine need of large quantities of radioactivity. The 4G10 monoclonal anti-phosphotyrosine antibody (Cat. # 05-321) has been widely recognized as a highly specific and sensitive. This antibody is suitable for all applications involving protein tyrosine phosphorylation. The slightly less sensitive, but still highly regarded clone PY20 can be used as an alternative to the 4G10 product line.

特异性

Recognizes proteins containing phosphotyrosine residues, regardless of origin.

免疫原

Phosphotyrosine conjugated to carrier protein
Epitope: phosphotyrosine

应用

Research Sub Category
General Post-translation Modification
Research Category
Signaling
Anti-Phosphotyrosine Antibody, clone PY20, HRP Conjugate is a Mouse Monoclonal Antibody for the detection of Phosphotyrosine & has been validated in WB, ELISA.

质量

Routinely evaluated by western blot on RIPA lysates from insulin-treated human HEK293 cells.

目标描述

Varies

外形

Protein A purified
100 μL of protein A purified mouse IgG2b in PBS containing 0.05% Kathon^?and HRP stabilizing compounds. Sufficient for a minimum of 10 immunoblots, if used at the optimum concentration of 1:2000- 1:4000 in 10 mL blocking buffer. Liquid.

储存及稳定性

6 months at 2-8°C from date of shipment.
NOTE: DO NOT FREEZE. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

法律信息

Kathon is a registered trademark of Rohm and Haas Co.
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
偶联物	peroxidase conjugate
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	PY20, monoclonal
species reactivity (predicted by homology)	all
manufacturer/tradename	Upstate?
technique(s)	ELISA: suitable western blot: suitable
同位素/亚型	IgG2b
运输	wet ice

安全信息

象形图
警示用语：	Warning
危险声明	H315 - H317 - H319 - H411
预防措施声明	P261 - P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338
危险分类	Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
储存分类代码	12 - Non Combustible Liquids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable