Anti-PCNA Antibody, clone PC10

半岛bd体育手机客户端 说明

一般描述

Proliferating cell nuclear antigen (PCNA) is a heterotrimeric nuclear protein that functions as a clamp on replicating DNA. In cases of DNA damage and failure of the DNA repair pathways, PCNA mediates translesion DNA synthesis, a DNA damage tolerance (DDT) pathway, which allows replication to progress in spite of large lesions to DNA which may be caused by replication stress, ionizing radiation, spontaneous DNA damage, among other factors. PCNA primarily recruits low-fidelity DNA polymerase enzymes that are capable of elongating DNA over lesion sites. PCNA is regulated by cycles of ubiquitination, depending on the DDT pathway involved: PCNA is monoubiquitinated by Rad6–Rad18; polyubiquitinated by the Mms2-Ubc13-Rad5; or sumoylated by the Ubc9-Siz1 complex.

免疫原

Recombinant protein corresponding to human PCNA.

应用

Research Sub Category
Cell Cycle, DNA Replication & Repair
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected PCNA in human colon adenocarcinoma tissues and human tonsil tissues.

Immunofluorescence Analysis: A 1:1,500 dilution from a representative lot detected PCNA in human lymph nodes and in human colon adenocarcinoma cells.
Research Category
Epigenetics & Nuclear Function
Use Anti-PCNA Antibody, clone PC10 (Mouse Monoclonal Antibody) validated in WB, IHC, IF to detect PCNA also known as Proliferating cell nuclear antigen, PCNA, Cyclin.

质量

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.01 ?g/mL of this antibody recognized PCNA in 10 ?g of HeLa cell lysate.

目标描述

~29 kDa observed

外形

Format: Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
HeLa cell lysate

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	PC10, monoclonal
species reactivity	human
technique(s)	immunofluorescence: suitable immunohistochemistry: suitable western blot: suitable
同位素/亚型	IgG2aκ
NCBI登记号	NP_002583
UniProt登记号	P12004
运输	wet ice

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable