Anti-Mouse CD5 (53-7.3) ColorWheel^?Dye-Ready mAb

半岛bd体育手机客户端 说明

一般描述

ColorWheel^?technologyis a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel^?antibody with any ColorWheel^?dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel^?antibody and dye is lyophilized for long long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel^?technologyrequires both ColorWheel^?antibody and ColorWheel^?dyefor Flow Cytometry application.

特异性

Clone 53-7.3 is a rat monoclonal antibody that detects murine CD5. It binds to a monomorphic epitope on CD5.

免疫原

Murine thymus/spleen cells

应用

Quality Control Testing:

Evaluated by Flow Cytometry in mouse splenocytes.

Flow Cytometry Analysis: Staining of one million mouse splenocytes was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1032, Anti-Mouse CD5 (53-7.3) ColorWheel^?Dye-Ready mAb and Cat. No. CWD-PE ColorWheel^?Antibody-Ready Phycoerythrin (PE) Dye or an equivalent amount of PE-conjugated Rat IgG2a isotype control.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

相容性

Requires ColorWheel^?Dye (Sold Separately)

目标描述

T-cell surface glycoprotein CD5 (UniProt: P13379; also known as Lymphocyte antigen 1, Ly-1, Lyt-1, CD5) is encoded by the Cd5 (also known as Ly-1) gene (Gene ID: 12507) in murine species. CD5 is a single-pass type I membrane protein that is synthesized with a signal peptide (aa 1-23), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 24-371), a transmembrane domain (aa 372-401), and a cytoplasmic domain (aa 402-494). In its extracellular domain it contains three scavenger receptor cysteine-rich (SRCR) domains. It is expressed on the surface of T cells bearing the β heterodimer T cell receptor (TCR) and a subset of murine B cells known as B-1a cells. It is either not present or is present at low density on the T cell subset. CD5 serves as a transmembrane receptor that regulates T cell proliferation, functions, and development. CD72 is recognized as one of the ligands for CD5. It is considered as an activation marker of T cells. Its expression is reported to increase according to the magnitude of the signal delivered by the TCR. It has been reported that the absence of CD5 in mice can enhance signaling and activation of double positive thymocytes induced by TCR stimulation, indicating that it acts as a negative regulator of TCR signaling in T cells. In mature, peripheral T cells, CD5 is reported to be present in lipid rafts of the T cell surface, where it promotes CD3 redistribution into rafts and upregulates ZAP-70 and LAT activation. (Ref.: Voisinne, G., et al. (2018). Front. Immunol. 9; 2900; Gary-Gouy, H., et al. (1997). J. Immunol. 159(8); 3739-3747).

外形

Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

重悬

1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommend storage of lyophilized product at 2-8℃. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL of PBS. Protect from light.

法律信息

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

半岛bd体育手机客户端 性质

质量水平	200
生物来源	rat
抗体形式	purified antibody
antibody product type	primary antibodies
克隆	53-7.3, monoclonal
半岛bd体育手机客户端 线	ColorWheel?
形式	lyophilized
分子量	calculated mol wt 53.85 kDa
species reactivity	mouse
包装	antibody small pack of 25 μL
technique(s)	flow cytometry: suitable
同位素/亚型	IgG2aκ
表位序列	Unknown
Protein ID登记号	NP_031676.3
UniProt登记号	P13379
相容性	for use with ColorWheel?Dyes (Required, sold separately)
运输	ambient
储存温度	2-8℃
Gene Information	mouse ... CD5(12507)

安全信息

储存分类代码	13 - Non Combustible Solids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable