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位置: 首页> 品牌> Sigma-Aldrich> Anti-Human CD64 (10.1) Colorwheel?Dye-Ready mAb

Anti-Human CD64 (10.1) Colorwheel?Dye-Ready mAb

品牌
Sigma-Aldrich
货号
CWA-1026
规格纯度
for use with ColorWheel?Dyes (Required, sold separately)
价格
1596.49 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
- +
半岛bd体育手机客户端 名称:
Anti-Human CD64 (10.1) Colorwheel ?Dye-Ready mAb
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

ColorWheel?technologyis a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel?antibody with any ColorWheel?dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. EachColorWheel?antibody and dye is lyophilized for long long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel?technologyrequires both ColorWheel?antibody and ColorWheel?dyefor Flow Cytometry application.

特异性

Clone 10.1 is a mouse monoclonal antibody that detects CD64 (FCgR1). It targets an epitope within the extracellular domain.

免疫原

Human rheumatoid synovial fluid cells and fibronectin purified monocytes.

应用

Quality Control Testing

Evaluated by Flow Cytometry in Human peripheral blood mononuclear cells (PBMC).

Flow Cytometry Analysis (FC): Staining of one million Human peripheral blood mononuclear cells (PBMC) was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1026, Anti-Human CD54 (10.1) ColorWheel?Dye-Ready mAb and Cat. No. CWD-PE ColorWheel?Antibody-Ready Phycoerythrin (PE) Dye or an equivalent amount PE-conjugated Mouse IgG1 isotype control.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

相容性

Requires ColorWheel?Dye (Sold Separately)

目标描述

High affinity immunoglobulin gamma Fc receptor I (UniProt: P12314; also known as IgG Fc receptor I, Fc-gamma RI, FcRI, Fc-gamma RIA, FcgammaRIa, CD64) is encoded by the FCGR1A (also known as FCG1, FCGR1, IGFR1) gene (Gene ID: 2209) in human. CD64 is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-15), which is subsequently cleaved off to produce the mature form that contains an extracellular domain (aa 16-292), a transmembrane domain (aa 293-313), and a cytoplasmic domain (aa 314-374). Within its extracellular domain are three Ig-like C2 type domains. Its expression has been reported in monocytes/macrophages. Expression of CD64 distinguishes conventional dendritic cells from monocyte-derived dendritic cells. It serves as a high-affinity receptor for the Fc domains of IgG (Fc?R) and plays a key role in linking the humoral and the cellular arms for the immune system. It binds human IgG1, IgG3, and IgG4 with a high affinity, but does not show any affinity for IgG2. It functions in both innate and adaptive immune responses. Along with the low affinity receptor (Fc?RII), it mediates a variety of biological responses, including endocytosis, phagocytosis, antibody-directed cellular cytotoxicity, and cytokine release. Clone 10.1 is shown to react with an epitope on Fc?RI near to the binding site for the Fc region of IgG. (Ref.: Harrison, PT., and Allen., (1998). Protein Engineering. 11 (3); 225-232; Dougherty, GJ., et al. (1987). Eur. J. Immunol. 17(10); 1453-1459).

外形

Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

重悬

1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommend storage of lyophilized product at 2-8℃. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL PBS. Protect from light.

法律信息

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

半岛bd体育手机客户端 性质

质量水平 200
生物来源 mouse
抗体形式 purified antibody
antibody product type primary antibodies
克隆 10.1, monoclonal
半岛bd体育手机客户端 线 ColorWheel?
形式 lyophilized
分子量 calculated mol wt 42.63 kDa
species reactivity human
包装 antibody small pack of 25 μL
technique(s) flow cytometry: suitable
同位素/亚型 IgG1κ
表位序列 Extracellular domain
Protein ID登记号 NP_001365740.1
UniProt登记号 P12314
相容性 for use with ColorWheel?Dyes (Required, sold separately)
运输 ambient
储存温度 2-8℃
Gene Information mouse ... FCGR1A(2209)

安全信息

储存分类代码 11 - Combustible Solids
WGK WGK 2
闪点(F) Not applicable
闪点(C) Not applicable

Sigma-Aldrich

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