Anti-Human CD56 (MY31) Colorwheel^?Dye-Ready mAb

半岛bd体育手机客户端 说明

一般描述

ColorWheel^?technologyis a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel^?antibody with any ColorWheel^?dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. EachColorWheel^?antibody and dye is lyophilized for long long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel^?technologyrequires both ColorWheel^?antibody and ColorWheel^?dyefor Flow Cytometry application.

特异性

Clone MY31 is a mouse monoclonal antibody that detects Neural cell adhesion molecule 1 (CD56).

免疫原

KG1a leukemia cells

应用

Quality Control Testing:

Evaluated by Flow Cytometry in Human peripheral blood mononuclear cells (PBMC).

Flow Cytometry Analysis (FC): Staining of one million Human peripheral blood mononuclear cells (PBMC) was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1067, Anti-Human CD56 (MY31) ColorWheel^?Dye-Ready mAb and Cat. No. CWD-PE ColorWheel^?Antibody-Ready Phycoerythrin (PE) Dye or an equivalent amount of PE-conjugated Mouse IgG1 isotype control.

Evaluated by Flow Cytometry in Human NK cells.

Staining of one million Human NK cells was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1067, Anti-Human CD56 (MY31) ColorWheel^?Dye-Ready mAb and Cat. No. CWD-PE ColorWheel^?Antibody-Ready Phycoerythrin (PE) Dye (yellow histogram) or an equivalent amount of PE-conjugated Mouse IgG1 isotype control.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

相容性

Requires ColorWheel^?Dye (Sold Separately)

目标描述

Neural cell adhesion molecule 1 (UniProt: P13591; also known as N-CAM-1, NCAM-1, CD56) is encoded by the NCAM1 (also known as NCAM) gene (Gene ID: 4684) in human. CD56 is a member of the immunoglobulin superfamily that is engaged in both so-called homophilic and heterophilic interactions. It is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-19), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 20-718), a transmembrane domain (aa 719-739), and a cytoplasmic domain (aa 740-858). It also contains five Ig-like C2-type domains and two fibronectin type-III domains. CD56 is expressed by neurons, glia, skeletal muscle, and natural killer (NK) cells. It is involved in neuron-neuron adhesion, neurite fasciculation, and outgrowth of neurite. It is considered as the archetypal phenotypic marker for natural killer cells, but is also expressed in many other immune cells, including ab T cells, ?g T cells, dendritic cells, and monocytes. It is also shown to play a role in the fungus mediated NK cell activation and blocking of CD56 surface receptor reduced fungal mediated NK cell activation and reduced cytokine secretion. (Ref.: Ziegler, S., et al. (2017). Sci. Rep. 7; Article 6138; Van Acker, HH., et al. (2017). Front. Immunol. 8; Article 892).

外形

Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

重悬

1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommend storage of lyophilized product at 2-8℃. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL of PBS. Protect from light.

法律信息

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

半岛bd体育手机客户端 性质

质量水平	200
生物来源	mouse
抗体形式	purified antibody
antibody product type	primary antibodies
克隆	MY31, monoclonal
半岛bd体育手机客户端 线	ColorWheel?
形式	lyophilized
分子量	calculated mol wt 94.57 kDa
species reactivity	human
species reactivity (predicted by homology)	nonhuman primates
包装	antibody small pack of 25 μL
technique(s)	flow cytometry: suitable
同位素/亚型	IgG1κ
表位序列	Unknown
Protein ID登记号	NP_000606.3
UniProt登记号	P13591
相容性	for use with ColorWheel?Dyes (Required, sold separately)
运输	ambient
储存温度	2-8℃
Gene Information	human ... NCAM1(4684)

安全信息

储存分类代码	13 - Non Combustible Solids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable