Anti-Human CD49f (4F10) Colorwheel^?Dye-Ready mAb

半岛bd体育手机客户端 说明

一般描述

ColorWheel^?technologyis a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel^?antibody with any ColorWheel^?dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. EachColorWheel^?antibody and dye is lyophilized for long long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel^?technologyrequires both ColorWheel^?antibody and ColorWheel^?dyefor Flow Cytometry application.

特异性

Clone 4F10 is a mouse monoclonal antibody that detects human integrin a6 (CD49f).

免疫原

SW1222 human colorectal cells.

应用

Quality Control Testing

Evaluated by Flow Cytometry in Human peripheral blood mononuclear cells (PBMC).

Staining of one million Human peripheral blood mononuclear cells (PBMC) was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1072, Anti-Human CD49f (4F10) ColorWheel^?Dye-Ready mAb and Cat. No. CWD-PE ColorWheel^?Antibody-Ready Phycoerythrin (PE) Dye or an equivalent amount of PE-conjugated Mouse IgG2b isotype control.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

相容性

Requires ColorWheel^?Dye (Sold Separately)

目标描述

Integrin alpha-6 (UniProt: P23229; also known as CD49 antigen-like family member F, VLA-6, CD49f) is encoded by the ITGA6 gene (Gene ID: 3655) in human. Integrins are heterodimeric integral membrane proteins composed of an a subunit and a b subunit that function in cell surface adhesion and signaling. They contain a large extracellular domain responsible for ligand binding, a single transmembrane domain, and a cytoplasmic domain. The exact combination of various a- and b-subunits dictates the binding specificity of integrins to different ECM components. Although both subunits are required for adhesion, the binding specificity primarily depends on the extracellular portion of the a-subunit. The structural and functional diversity of the integrin family is based upon the pairing abilities of the individual a and b subunits. Integrins are not constitutively active and their activation from a low ligand-binding affinity state to high ligand-binding affinity state requires conformational change that can originate from either from their cytoplasmic or extracellular interactions. The recognition site for most integrins that bind the ECM consists of an RGD (arginine-glycine-aspartic acid) sequence. Integrin a-6 is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-23), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 24-1050), a transmembrane domain (aa 1051-1076), and a cytoplasmic domain (aa 1077-1130). The mature form can be further processed into integrin a-6 heavy chain (aa 24-938), integrin a-6 light chain ( aa 942-1130), and a processed integrin a6 (aa 636-1130). Integrin a6 can heterodimerize with b1 to form a receptor for laminin on platelets. a6/b1 is also present in oocytes and is involved in sperm-egg fusion. Integrin a6/b4 heterodimer is a receptor for laminin in epithelial cells and plays a role in the hemidesmosome. It also binds to IGF-1 and IGF-2 and these binding are shown to be essential for IGF-2 signaling.

外形

Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

重悬

1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommend storage of lyophilized product at 2-8℃. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL PBS. Protect from light.

法律信息

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

半岛bd体育手机客户端 性质

质量水平	200
生物来源	mouse
抗体形式	purified antibody
antibody product type	primary antibodies
克隆	4F10, monoclonal
半岛bd体育手机客户端 线	ColorWheel?
形式	lyophilized
分子量	calculated mol wt 126.61 kDa
species reactivity	human
包装	antibody small pack of 25 μL
technique(s)	flow cytometry: suitable
同位素/亚型	IgG2bκ
表位序列	Unknown
Protein ID登记号	NP_000201.2
UniProt登记号	P23229
相容性	for use with ColorWheel?Dyes (Required, sold separately)
运输	ambient
储存温度	2-8℃
Gene Information	mouse ... ITGA6(3655)

安全信息

储存分类代码	11 - Combustible Solids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable