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位置: 首页> 品牌> Sigma-Aldrich> Anti-Human CD20 (2H7) ColorWheel?Dye-Ready mAb

Anti-Human CD20 (2H7) ColorWheel?Dye-Ready mAb

品牌
Sigma-Aldrich
货号
CWA-1036
规格纯度
for use with ColorWheel?Dyes (Required, sold separately)
价格
1596.49 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
- +
半岛bd体育手机客户端 名称:
Anti-Human CD20 (2H7) ColorWheel ?Dye-Ready mAb
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

ColorWheel?technologyis a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel?antibody with any ColorWheel?dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. EachColorWheel?antibody and dye is lyophilized for long long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel?technologyrequires both ColorWheel?antibody and ColorWheel?dyefor Flow Cytometry application.

特异性

Clone 2H7 is a mouse monoclonal antibody that detects CD20. It targets an epitope within 16 amino acids from the second extracellular domain.

免疫原

Human tonsillar B cells.

应用

Quality Control Testing:

Evaluated by Flow Cytometry in Raji cells with Fc block.

Staining of one million Human Raji cells with Fc block was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1036, Anti-Human CD20 (2H7) ColorWheel?Dye-Ready mAb and Cat. No. CWD-PE ColorWheel?Antibody-Ready Phycoerythrin (PE) Dye or the equivalent amount of Cat. No. CWA-1010 Mouse IgG2b isotype control and Cat. No. CWD-PE ColorWheel?Antibody-Ready Phycoerythrin (PE) Dye.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

相容性

Requires ColorWheel?Dye (Sold Separately)

目标描述

B-lymphocyte antigen CD20 (UniProt: P11836; also known as B-lymphocyte surface antigen B1, Bp35, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1, CD20) is encoded by the MS4A1 (also known as CD20) gene (Gene ID: 931) in human. CD20 is a multi-pass homotetrameric, non-glycosylated membrane protein that is expressed on the surface of normal and malignant B-lymphocytes. CD20 expression is reportedly initiated at the pre-B cell stage of development and remains present until terminal differentiation into a plasma cell at which time it is lost. It has three cytoplasmic domains, four transmembrane domains, and two extracellular domains. Its both N- and C-termini reside within the cytoplasm. CD20 plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes. It functions as a store-operated calcium channel component. It undergoes phosphorylation by PKC on serine and threonine residues in resting cells and these phosphorylations are reported to be higher in proliferating malignant B cells than in resting cells. CD20 is considered as an important therapeutic target in the treatment of B cell malignancies and various antibodies have been developed that bind to different epitopes . (Ref.: Pavlasova, G., and Mraz, M. (2020). Haematologica. 105(6); 1494-1506; Klein, C., et al. (2013). MAbs. 5(1); 22 33).

外形

Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

重悬

1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommend storage of lyophilized product at 2-8℃. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL of PBS. Protect from light.

法律信息

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

半岛bd体育手机客户端 性质

质量水平 200
生物来源 mouse
抗体形式 purified antibody
antibody product type primary antibodies
克隆 2H7, monoclonal
半岛bd体育手机客户端 线 ColorWheel?
形式 lyophilized
分子量 calculated mol wt 33.08 kDa
species reactivity human
species reactivity (predicted by homology) nonhuman primates
包装 antibody small pack of 25 μL
technique(s) flow cytometry: suitable
同位素/亚型 IgG2bκ
表位序列 Extracellular domain
Protein ID登记号 NP_690606.1
UniProt登记号 P11836
相容性 for use with ColorWheel?Dyes (Required, sold separately)
运输 ambient
储存温度 2-8℃
Gene Information human ... MS4A1;CD20(931)

安全信息

储存分类代码 11 - Combustible Solids
WGK WGK 3
闪点(F) Not applicable
闪点(C) Not applicable

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