半岛bd体育手机客户端 说明
一般描述
ColorWheel?technologyis a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel?antibody with any ColorWheel?dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel?antibody and dye is lyophilized for long long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel?technologyrequires both ColorWheel?antibody and ColorWheel?dyefor Flow Cytometry application.
特异性
Clone 28 is a mouse monoclonal antibody that detects human CD15.
免疫原
Monocytes separated from other peripheral leukocytes on fibronectin plates.
应用
Quality Control Testing:
Evaluated by Flow Cytometry in human blood granulocytes .
Flow Cytometry Analysis: Staining of one million Whole human blood granulocytes was performed using 5 μl of a 1:1 mixture of Cat. No. CWA-1001, Anti-CD15 Antibody (28) ColorWheel?Mouse mAb and CW-PE ColorWheel?activated Phycoerythrin (PE) Dye or an equivalent amount of Mouse IgM isotype control.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
相容性
Requires ColorWheel?Dye (Sold Separately)
目标描述
Alpha-(1,3)-fucosyltransferase 4 (UniProt: P22083; also known as ELAM-1 ligand fucosyltransferase, Fucosyltransferase 4, Fucosyltransferase IV, Fuc-TIV, FucT-IV, Galactoside 3-L-fucosyltransferase, CD15) is encoded by the FUT4 (also known as ELFT, FCT3A) gene (Gene ID: 2526) in human. CD15 is a single-pass type II membrane protein of the glycosyltransferase 10 family. It contains a cytoplasmic domain (aa 1-147), a transmembrane domain (aa 148-172), and a lumenal domain (aa 173-530). It consists of a trisaccharide with the structure Gal-β(1-4)-(Fuc- (1-3))-GlcNAc and is related to another structure, sialyl-CD15 (NeuNAc- (2-3)-Gal-β(1-4)-(Fuc- (1-3))-GlcNAc; CD15s or sLex, where s refers to sialylated ), which differs only by the addition of a sialic acid linkage to the galactose in the core Lex trisaccharide. CD15 catalyzes alpha-1,3 glycosidic linkages involved in the expression of Lewis X/SSEA-1 and VIM-2 antigens. It is shown to be a distinguishing marker for human myeloid cells, and it mediates neutrophil adhesion to dendritic cells. It is also involved in neutrophil functions, such as cell-cell interactions, phagocytosis, stimulation of degranulation, and respiratory burst. It is also considered as an important marker for neoplastic Reed Sternberg cells and can be useful for the diagnosis of Hodgkin s lymphoma. (Ref.: Gadhoum, SZ., and Sackstein, R (2008). Nat. Chem. Biol. 4(12); 751-757; Nakayama, F., et al. (2001).Glycobiol. Extracellular Matrices. 276(19); 16100-16106).
外形
Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.
重悬
1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
储存及稳定性
Recommend storage of lyophilized product at 2-8℃. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL of PBS. Protect from light.
法律信息
ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
半岛bd体育手机客户端 性质
质量水平 | 200 |
生物来源 | mouse |
抗体形式 | purified antibody |
antibody product type | primary antibodies |
克隆 | 28, monoclonal |
半岛bd体育手机客户端 线 | ColorWheel? |
形式 | lyophilized |
分子量 | calculated mol wt 59.08 kDa |
species reactivity | human |
包装 | antibody small pack of 25 μL |
technique(s) | flow cytometry: suitable |
同位素/亚型 | IgMκ |
表位序列 | Unknown |
Protein ID登记号 | NP_002024.1 |
UniProt登记号 | P22083 |
相容性 | for use with ColorWheel?Dyes (Required, sold separately) |
运输 | ambient |
储存温度 | 2-8℃ |
Gene Information | human ... FUT4(2526) |
安全信息
储存分类代码 | 11 - Combustible Solids |
WGK | WGK 2 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich