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位置: 首页> 品牌> Sigma-Aldrich> Anti-Human CD11c (3.9) ColorWheel?Dye-Ready mAb

Anti-Human CD11c (3.9) ColorWheel?Dye-Ready mAb

品牌
Sigma-Aldrich
货号
CWA-1056
规格纯度
for use with ColorWheel?Dyes (Required, sold separately)
价格
1596.49 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
- +
半岛bd体育手机客户端 名称:
Anti-Human CD11c (3.9) ColorWheel ?Dye-Ready mAb
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

ColorWheel?technologyis a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel?antibody with any ColorWheel?dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. EachColorWheel?antibody and dye is lyophilized for long long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel?technologyrequires both ColorWheel?antibody and ColorWheel?dyefor Flow Cytometry application.

特异性

Clone 3.9 is a mouse monoclonal antibody that detects Integrin alpha-X (CD11c). It targets an epitope within the I-domain from the N-terminal half.

免疫原

Human monocytes and synovial cells.

应用

Quality Control Testing:

Evaluated by Flow Cytometry in Human peripheral blood mononuclear cells (PBMC).

Staining of one million human peripheral blood mononuclear cells (PBMC) was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1056, Anti-Human CD11c (3.9) ColorWheel?Dye-Ready mAb and Cat. No. CWD-PE ColorWheel?Antibody-Ready Phycoerythrin (PE) Dye or the equivalent amount of PE-conjugated Mouse IgG1 isotype control.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

相容性

Requires ColorWheel?Dye (Sold Separately)

目标描述

Integrin alpha-X (UniProt: P20702; also known as CD11 antigen-like family member C; Leu M5; Leukocyte adhesion glycoprotein p150,95 alpha chain; Leukocyte adhesion receptor p150-95; CD11c) is encoded by the ITGAX (also known as CD11C) gene (Gene ID: 3687) in human. CD11c is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-19), which is subsequently cleaved off to generate the mature form that contains a long extracellular domain (aa 20-1107), a transmembrane domain (aa 1108-1128), and a cytoplasmic domain (aa 1129-1163). CD11c is predominantly expressed in macrophages, monocytes, and granulocytes and along with integrin β2 it serves as a receptor for fibrinogen. It recognizes the sequence G-P-R in fibrinogen. The αXβ2 heterodimer binds many ligands, including collagen type 1, iC3b, fibrinogen, ICAM-1, and lipopolysaccharide. It is reported to mediate cell-cell interaction during inflammatory responses and is especially important in monocyte adhesion and chemotaxis. The I domains of CD11 are responsible for the binding of ligands and have a unique structure with 6-7 α-helixes and 6β sheets with interconnecting loops. In CD11c the loops on the I domain are involved in fibrinogen binding and loops α3-α4 and βD-α5 are reported to be more important for recognition of fibrinogen. (Ref.: Vorup-Jensen, T., et al. (2003). Proc. Natl. Acad. Sci. USA. 100(4); 1873-1878; Choi, J., and Nham, S-U. (2002). Biochem. Biophys. Res. Commun. 292(3); 756-760).

外形

Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

重悬

1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommend storage of lyophilized product at 2-8℃. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL of PBS. Protect from light.

法律信息

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

半岛bd体育手机客户端 性质

质量水平 200
生物来源 mouse
抗体形式 purified antibody
antibody product type primary antibodies
克隆 3.9, monoclonal
半岛bd体育手机客户端 线 ColorWheel?
形式 lyophilized
分子量 calculated mol wt 127.38 kDa
species reactivity human
species reactivity (predicted by homology) nonhuman primates
包装 antibody small pack of 25 μL
technique(s) flow cytometry: suitable
同位素/亚型 IgG1κ
表位序列 Unknown
Protein ID登记号 NP_001273304.1
UniProt登记号 P20702
相容性 for use with ColorWheel?Dyes (Required, sold separately)
运输 ambient
储存温度 2-8℃
Gene Information human ... ITGAX;CD11C(3687)

安全信息

储存分类代码 11 - Combustible Solids
WGK WGK 3
闪点(F) Not applicable
闪点(C) Not applicable

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