Anti-CREB Antibody

半岛bd体育手机客户端 说明

一般描述

CREB is a beta ZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth and neuronal differentiation in certain neuronal populations. Additionally, CREB signaling is involved in learning and memory in several organisms. CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca2+ and stress signaling. Some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV and MAPKAPK-2.

特异性

Human cyclic AMP responsive element binding protein-1 (CREB-1, p43). By Western blot it shows a major band at 46 kDa when assayed against a lysate of 1 x 106 Raji B cells. Minor bands at 75 kDa and 43 kDa may be noted. Partial reactivity to CREM-1 protein. May react non-specifically with other proteins.

免疫原

CREB-1 (p43) peptide corresponding to the N-terminal (aa 5-45) of human protein conjugated to KLH.

应用

Use Anti-CREB Antibody (Rabbit Polyclonal Antibody) validated in ELISA, EMSA, IP, WB to detect CREB also known as CREB-1, cAMP-response Element-binding Protein-1.
Immunoblot

Immunoprecipitation

ELISA

Supershift assay: 0.5-1.0 μL per assay.

Optimal dilutions must be determined by the end user.

联系

Replaces: 04-218

外形

Purified immunoglobulin. Liquid (sterile filtered) in 0.02M Potassium Phosphate buffer containing 0.15 M Sodium Chloride, pH 7.2 with 0.01% sodium azide.
Format: Purified

储存及稳定性

Maintain frozen at -20°C in undiluted aliquots for up to six months. Avoid repeated freeze/thaw cycles.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	100
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	polyclonal
species reactivity	human, mouse, rat
manufacturer/tradename	Chemicon?
technique(s)	ELISA: suitable electrophoretic mobility shift assay: suitable immunoprecipitation (IP): suitable western blot: suitable
NCBI登记号	NM_004379.2 NM_134442.2
UniProt登记号	P16220
运输	wet ice
Gene Information	human ... CREB1(1385)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable