半岛bd体育手机客户端 说明
一般描述
Tubulins, major components of the cellular cytoskeleton, form microtubules that are critical for cellular structure, migration, cell division and intracellular trafficking. There are at least three types of tubulins, alpha, beta and gamma. Each has a molecular weight of approximately 55 kDa. Microtubules are large structures (300 angstrom diameter) composed of alternating alpha and beta subunits. Compounds that block microtuble formation, such as Taxol and the vinca alkaloids, inhibit cell division and are effective anti-cancer agents.
特异性
Recognizes α-Tubulin, clone DM1A, Mr 50-60 kDa.
免疫原
Epitope: The epitopehas been mapped to amino acids 426-450 (AALEKDYEEVGVDSV-EGEGEEEGEE).
Purified human brain α-Tubulin. The epitope recognized by this antibody has been mapped to amino acids 426-450 (AALEKDYEEVGVDSV-EGEGEEEGEE).
应用
Western Blot Analysis:
2 ?g/mL of a previous lot detected α-Tubulin in RIPA lysates from A431 cells.
Immunocytochemistry:
2 ?g/mL of a previous lot showed positive immunostaining for α-Tubulin in HeLa cells.
Anti-α-Tubulin Antibody, clone DM1A, Alexa Fluor 488 conjugate is an antibody against α-Tubulin for use in FC, IC, IF & WB.
质量
Routinely evaluated by Flow Cytometry.
Flow Cytometry:
0.2 μg of this lot detected α-Tubulin in fixed and permeabilized rat L6, Jurkat, and A431 cells.
目标描述
50-60 kDa
外形
Alexa Fluor?488 conjugated, purified mouse monoclonal IgG1 in buffer containing PBS containing 1% BSA, 0.05% Tween
Liquid at 2-8°C.
Protein G Purified
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
Do Not Freeze. Do not store the material diluted.
For maximum recovery of product, centrifuge original vial prior to removing cap.
分析说明
Control
Included Negative Control: Catalog # 16-240, Normal Mouse IgG, Alexa Fluor?488-conjugate.
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
ALEXA FLUOR is a registered trademark of Life Technologies
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Alexa Fluor?is a registered trademark of Molecular Probes, Inc.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
半岛bd体育手机客户端 性质
| 质量水平 | 100 |
| 生物来源 | mouse |
| 偶联物 | ALEXA FLUOR?488 |
| 抗体形式 | purified antibody |
| antibody product type | primary antibodies |
| 克隆 | DM1A, monoclonal |
| species reactivity | mouse, guinea pig, pig, human, rat, avian, bovine |
| manufacturer/tradename | Upstate? |
| technique(s) | flow cytometry: suitable immunocytochemistry: suitable immunofluorescence: suitable western blot: suitable |
| 同位素/亚型 | IgG1 |
| NCBI登记号 | NP_006000 |
| UniProt登记号 | Q71U36 |
| 运输 | wet ice |
| Gene Information | human ... TUBA1A(7846) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 2 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
