Anti-53BP1 Antibody, clone BP13

半岛bd体育手机客户端 说明

一般描述

P53-binding protein 1 (53BP1) plays a critical role in tumor suppression and is a putative substrate of ATM kinase. Upon DNA damage, it is phosphorylated and relocalizes to the presumptive sites of damage, specifically, double-strand breaks. This also suggests a role in DNA repair, and maintaining genomic stability. It is sequence specific transcription factor that plays a central role in the the response of mammalian cells to genotoxic stress. Also, 53BP1 localizes to kinetochores in mitotic cells, suggesting a potential function in mitotic checkpoint signaling.

特异性

53BP1

免疫原

Recombinant human 53BP1 proteins.

应用

Detect 53BP1 also known as p53 Binding Protein 1 with Anti-53BP1 Antibody, clone BP13 (Mouse Monoclonal Antibody) validated in IF & WB.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Western blot. Recognizes a protein of >250 kD. Suggested dilution buffer is TBST. Suggested blocking buffer is 5% non-fat dry milk.

Immunoprecipitation. Expected size in immunoblot is >250 kD. Suggested lysis buffer is NETN. Suggested capture agent is Protein A + rabbit anti-mouse linker antibody.

Optimal working dilutions must be determined by the end user.

外形

Purified immunoglobulin. Liquid in PBS, containing sodium azide.
Format: Purified

储存及稳定性

Maintain at 2-8°C in undiluted aliquots for up to 12 months from date of receipt.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	BP13, monoclonal
species reactivity	human
manufacturer/tradename	Chemicon?
technique(s)	immunofluorescence: suitable western blot: suitable
同位素/亚型	IgG1
NCBI登记号	NM_005657.1
UniProt登记号	Q12888
运输	wet ice

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable