半岛bd体育手机客户端 说明
一般描述
Integrin beta 1, also known as CD29, is a 130 kDa transmembrane glycoprotein that forms noncovalent complexes with various Integrin alpha subunits (including alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, and alpha 6) to form the functional receptors that bind to specific extracellular matrix proteins. Integrin receptors are involved in the regulation of a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor cell growth, and metastasis. These heterodimeric receptors bridge the cytoplasmic actin cytoskeleton with proteins present in the extracellular matrix and/or on adjacent cells. Interactions between integrins and the extracellular matrix lead to activation of signal transduction pathways and regulation of gene expression.
特异性
Reacts with Human Integrin Beta 1.
免疫原
Human keratinocytes derived from skin.
应用
Research Category
Cell Structure
Research Sub Category
Integrins
Immunoprecipitation:A representative lot of this antibody clone was used in immunoprecipitation.
Immunohistochemistry:A representative lot of this antibody clone was used in immunohistochemistry (acetone fixation, no paraffin embedding).
ELISA:A representative lot of this antibody was used in ELISA.
Immunocytochemistry:A representative lot of this antibody clone was used in immunocytochemistry (paraformaldehyde fixation at less than 4%).
Functional Activity Assay:A representative lot of this antibody clone was used in cell attachment assay of SV-HFO cells with a characteristic spread morphology. In the presence of function-blocking mAbs to β1 integrin (P5D2), the cells attached but no longer spread, and displayed a rounded morphology with many cytoplasmic projections (Iba, K. et al., 2000).
Anti-Integrin β1 Antibody, clone P5D2 (Azide Free) detects level of Integrin β1 & has been published & validated for use in ELISA, FC, IC, IH, IP, FUNC.
质量
Flow Cytometry Analysis:
4 ?g of the antibody was used to detect Integrin β1 in 1x10^6 A431 cells.
4 ?g of the antibody was used to detect Integrin β1 in 1x10^6 HeLa cells.
外形
Purified ascites in PBS without preservatives.
Format: Purified
Protein A purified
储存及稳定性
Store at -20°C in undiluted aliquots.
Avoid repeated freeze-thaw cycles.
分析说明
Control
Human tonsil, human skin tissue
A431 & HeLa Cells
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
半岛bd体育手机客户端 性质
质量水平 | 100 |
生物来源 | mouse |
抗体形式 | purified antibody |
antibody product type | primary antibodies |
克隆 | P5D2, monoclonal |
species reactivity | human |
manufacturer/tradename | Chemicon? |
technique(s) | ELISA: suitable flow cytometry: suitable immunocytochemistry: suitable immunohistochemistry: suitable immunoprecipitation (IP): suitable |
同位素/亚型 | IgG2bκ |
NCBI登记号 | NM_002211.2 NM_033666.1 NM_033667.1 NM_033668.1 NM_033669.1 NM_133376.1 |
UniProt登记号 | P05556 |
运输 | wet ice |
Gene Information | human ... ITGB1(3688) |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 2 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich