Anti-Integrin β1 Antibody, clone P4G11, azide free

半岛bd体育手机客户端 说明

特异性

Human integrin beta1. The epitope recognized by MAB1951Z is Ca2+ dependent.

免疫原

Human IL-1beta activated endothelial cells

应用

Research Category
Cell Structure
Immunohistochemistry: for use on acetone or mild paraformaldehyde fixed tissue (2-2.5% PFA, short time) frozen and 4% PFA paraffin embedded {Qin et al 2003 1:500, citrate HEIR treated}

Ca++ required for binding. Ca++ must be used in blocking, washing, and antibody incubation buffers.

Traditional formalin fixation is not recommended.

Immunocytochemistry: 2 ug/mL (for use on numerous cell lines as long as Ca2+ is present)

Immunoprecipitation: 5 μg/400 μL lysate (0.5% triton PBS plus Ca++)

FACS: 2-5μg per 10e6 cells, in the presence of Ca++.

Stimulation of cells: 2-10 μg/ml per 10e6 cells. Binding buffer must contain 1mM Ca++.

ELISA

Optimal working dilutions must be determined by end user.
Research Sub Category
Integrins
This Anti-Integrin β1 Antibody, clone P4G11, azide free is validated for use in ELISA, FC, IP, FUNC, FUNC, IC, IH for the detection of Integrin β1.

外形

Format: Purified
Sterile-filtered protein A purified immunoglobulin in 0.01M PBS, pH 7.1, containing no preservatives.

储存及稳定性

Maintain between 2 and 8°C for up to 6 months from date of receipt. Avoid freeze thaws.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	P4G11, monoclonal
species reactivity	rabbit, human, monkey
manufacturer/tradename	Chemicon?
technique(s)	ELISA: suitable flow cytometry: suitable immunocytochemistry: suitable immunohistochemistry: suitable immunoprecipitation (IP): suitable
同位素/亚型	IgG1
NCBI登记号	NM_002211.2 NM_033666.1 NM_033667.1 NM_033668.1 NM_033669.1 NM_133376.1
UniProt登记号	P05556
运输	wet ice
Gene Information	human ... ITGB1(3688)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable