半岛bd体育手机客户端 说明
一般描述
The development of the anti-phosphotyrosine, clone 4G10
特异性
Tyrosine-phosphorylated proteins from all species
免疫原
4G10
应用
Anti-Phosphotyrosine Antibody, 4G10 Platinum is an antibody against Phosphotyrosine for use in WB, IF, FC, IH, IP, IC, ELISA.
Research Sub Category
General Post-translation Modification
Research Category
Signaling
质量
Routinely evaluated by immunoblot on a modified RIPA lysate from EGF-treated human A431 carcinoma cells
目标描述
Dependent upon the molecular weight of the tyrosine phosphorylated protein being detected.
外形
Format: Purified
Protein G-Sepharose
A proprietary mixture of protein G purified mouse monoclonals 4G10
储存及稳定性
2 years at 2-8°C from date of shipment.
NOTE: DO NOT FREEZE.
For maximum recovery of the product, centrifuge the original vial prior to removing the cap. If the product has accidentally been frozen and thawed, spin it at 13,000 x g for 10 minutes at 2-8°C. Save the supernatant for application.
分析说明
Control
Suggested Positive Antigen Control: Cat.# 12-302, EGF-stimulated A431 cell lysate is provided as a free positive antigen control for western immunoblotting. Aliquot as desired, refreeze immediately, and store at –20?C. The lysate is stable for 6 months at –20?C.
法律信息
4G10 is a registered trademark of Upstate Group, Inc.
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
半岛bd体育手机客户端 性质
质量水平 | 100 |
生物来源 | mouse |
抗体形式 | purified antibody |
antibody product type | primary antibodies |
克隆 | 4G10, monoclonal |
species reactivity (predicted by homology) | all |
manufacturer/tradename | Upstate? |
technique(s) | ELISA: suitable flow cytometry: suitable immunocytochemistry: suitable immunofluorescence: suitable immunohistochemistry: suitable immunoprecipitation (IP): suitable western blot: suitable |
同位素/亚型 | IgG2b |
运输 | wet ice |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich