半岛bd体育手机客户端 说明

一般描述

Monoclonal Anti-V5 (mouse IgG1 isotype) is derived from the V5 hybridoma, produced by fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to amino acid residues GKPIPNPLLGLDST (95-108) of the P/V proteins of the Paramyxovirus SV5, conjugated to KLH. The conjugate is then extensively dialyzed to remove unbound Cy3 fluorophore. Monoclonal Anti-V5, Cy3 conjugate recognizes the V5 tag sequence on V5 tagged fusion proteins by immunocytochemistry.

特异性

识别 V5 标签(GKPIPNPLLGLDST)融合蛋白

应用

Many recombinant proteins have been engineered to express a short sequence derived from the V5 molecule known as V5-tag. This tag facilitates the detection, isolation and purification of the proteins. Monoclonal antibodies reacting specifically with V5 and conjugated to Cy3 may be useful in various immunotechniques, and also to identify the expression of a V5 tagged fusion proteinin situby immunofluorescence.

外形

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

法律信息

Cy3 is a trademark of Cytiva

基本信息

MDL编号	MFCD03457579
NACRES	NA.56

半岛bd体育手机客户端 性质

质量水平	200
生物来源	mouse
偶联物	CY3 conjugate
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	V5-10, monoclonal
浓度	~1 mg/mL
technique(s)	immunocytochemistry: 2.0-4.0 μg/mL
运输	wet ice
储存温度	2-8℃

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable