Caspase-10, Human, Recombinant,E. coli

半岛bd体育手机客户端 说明

一般描述

M.W. 11,000 and 18,000 (heterodimer).
Recombinant, human caspase-10 fused at the N- and C- termini to a His?Tag sequence and expressed inE. colias a single polypeptide. Subsequently undergoes spontaneous autoprocessing to yield the active enzyme. Useful for the study of enzyme regulation and kinetics, cleavage of target substrates, and inhibitor screening. MW: 11000 and 18000.

包装

3000 u in Plastic ampoule

警告

Toxicity: Standard Handling (A)

单位定义

One unit is defined as the amount of enzyme that will cleave 1.0 pmol Ac-IETD-pNA (Cat. No. 368057) per min at 25°C, pH 7.4.

外形

In 100 mM NaCl, 50 mM HEPES, 10 mM DTT, 1 mM EDTA, 10% glycerol, 0.1% CHAPS, 0.09% APO-10, pH 7.4.

重悬

Following initial thaw, aliquot and freeze (-70°C).

其他说明

Kang, J.J., et al. 1999.J. Biol. Chem.274,3189.
Ng, P.W., et al. 1999.J. Biol. Chem.274,10301.
Vincenz, C., and Dixit, V.M. 1997.J. Biol. Chem.272,6578.
Srinivasula, S.M., et al. 1996.Proc. Natl. Acad. Sci. USA93,14486.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

半岛bd体育手机客户端 性质

质量水平	100
测定	≥95% (SDS-PAGE)
形式	liquid
specific activity	≥150,000 units/mg protein ≥50,000 units/mL
manufacturer/tradename	Calbiochem?
储存条件	OK to freeze avoid repeated freeze/thaw cycles
运输	wet ice
储存温度	?70℃

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 2