N-Glycosidase F,Elizabethkingia meningosepticum

半岛bd体育手机客户端 说明

一般描述

Native N-Glycosidase F fromElizabethkingia meningosepticum. Catalyzes the hydrolysis of asparagine-linked high mannose, as well as hybrid and complex oligosaccharides from glycoproteins. This enzyme will not cleave oligosaccharides containing α1-3-linked core fucose commonly found on plant glycoproteins. N-Glycosidase F will also fail to cleave if the asparagine to which the oligosaccharide is attached is not peptide-bonded to at least one amino acid residue at both the amino and carboxy termini.
Native N-Glycosidase F fromElizabethkingia meningosepticum. Catalyzes the hydrolysis of asparagine-linked high mannose, as well as hybrid and complex oligosaccharides from glycoproteins. This enzyme will not cleave oligosaccharides containing α1,3-linked core fucose commonly found on plant glycoproteins. N-Glycosidase F will also fail to cleave if the asparagine to which the oligosaccharide is attached is not peptide-bonded to at least one amino acid residue at both the amino and carboxy termini.

包装

100 u in Plastic ampoule

警告

Toxicity: Standard Handling (A)

单位定义

One unit is defined as the amount of enzyme that will catalyze the release of N-linked oligosaccharides from 1.0 nmol denatured ribonuclease B per min at 37°C, pH 7.5. Cleavage is monitored by SDS-PAGE. One unit of N-Glycosidase F is equal to 1 IUB milliunit.

外形

In 50 mM NaCl, 20 mM Tris-HCl, 1 mM EDTA, pH 7.5.

其他说明

Means, R.E. and Desrosiers, R.C. 2000.J. Virol.74,11181.
Fan, J.Q., and Lee, Y.C. 1997.J. Biol. Chem.272,27058.
Tarentino, A.L., and Plummer, T.H. 1994.Methods Enzymol.230,44.
Trimble, R.B., and Tarentino, A.L. 1991.J. Biol. Chem.266,1646.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

基本信息

酶学委员会(EC)编号	3.5.1.52 ( BRENDA | IUBMB )
MDL编号	MFCD00131222

半岛bd体育手机客户端 性质

形式	liquid
质量水平	100
specific activity	≥20,000 units/mg protein ≥4500 units/mL
manufacturer/tradename	Calbiochem?
储存条件	do not freeze
异质活性	Proteases, none detected
运输	wet ice
储存温度	2-8℃

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable