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Elastase, Porcine Pancreas, High Purity, Crystallized

品牌
Sigma-Aldrich
CAS
39445-21-1
货号
324682
规格纯度
Elastase, Porcine Pancreas, High Purity, Crystallized, CAS 39445-21-1, is a serine protease that hydrolyzes proteins and peptides, especially at bonds adjacent to neutral amino acid residues.
价格
6441 *本价格含增值税费
促销
服务
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数量
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半岛bd体育手机客户端 名称:
39445-21-1
Elastase, Porcine Pancreas, High Purity, Crystallized
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Native elastase from porcine pancreas. Catalyzes the hydrolysis of proteins and peptides (especially at bonds adjacent to neutral amino acid residues), including albumin, casein, denatured collagen, elastin, fibrin, and hemoglobin, and of a number of synthetic substrates containing aspartic acid, glutamic acid, phenylalanine, or tyrosine. Preferentially cleaves peptide bonds at the carbonyl end of amino acid residues with small hydrophobic side chains, such as glycine, valine, leucine, isoleucine, and particularly alanine. Inhibited by DFP, elastinal, and α2-macroglobulin. Has an optimal pH of 7.8-8.5; pI = 9.5. Note: Elastase tends to adhere to glass. Hence, use of siliconized glassware is recommended.
Native elastase from porcine pancreas. A serine protase that catalyzes the hydrolysis of proteins and peptides (especially at bonds adjacent to neutral amino acid residues), including albumin, casein, denatured collagen, elastin, fibrin, and hemoglobin and of a number of synthetic substrates containing aspartic acid, phenylalanine, or tyrosine. Inhibited by DFP, elastinal, and α2-macroglobulin.

包装

1000, 250 u in Plastic ampoule

警告

Toxicity: Standard Handling (A)

单位定义

One unit is defined as the amount of enzyme that will hydrolyze 1.0 mol of Suc-Ala-Ala-Pro-Abu-pNA (Cat no.324699) per min at 25℃, pH 8.0.

外形

Lyophilized from 50 mM Trehalose, 1 mM Acetic Acid.

重悬

Following reconstitution, elastase may be stored at 4°C at pH 6.0 for long term use. If incubated at room temperature at or near its optimum pH, elastase rapidly autolyzes to a mixture of peptides. Stock solutions are stable for up to 2 months at 4°C, pH 6.0.

其他说明

Largman, C. et al. 1976.Biochemestry15,2491.
Mandl, I. 1962.Methods in Enzymol.5,665.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

基本信息

酶学委员会(EC)编号 3.4.21.36 ( BRENDA | IUBMB )
MDL编号 MFCD00130998

半岛bd体育手机客户端 性质

质量水平 100
形式 lyophilized
specific activity ≥25 units/mg protein
manufacturer/tradename Calbiochem?
储存条件 OK to freeze
desiccated
溶解性 distilled water: 10 mg/mL
异质活性 Chymotrypsin ≤0.2%
运输 ambient
储存温度 ?20℃

安全信息

象形图 GHS07GHS08
警示用语: Danger
危险声明 H315 - H319 - H334 - H335
预防措施声明 P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
危险分类 Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
靶器官 Respiratory system
储存分类代码 11 - Combustible Solids
WGK WGK 3

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