L-Leucine Dehydrogenase,Bacillus cereus

半岛bd体育手机客户端 说明

一般描述

pH optimum 10.5-10.8 (L-leucine substrate) and 9.4 (α-ketoisocaproate substrate), temperature optimum 65°C.
Native L-leucine dehydrogenase fromBacillus cereus. Catalyzes the NAD⁺-dependent conversion of L-leucine to α-ketoisocaproate. Suitable for the production of L-tertiary leucine amino acids.
Native L-leucine dehydrogenase fromBacillus cereus. Purified enzyme that catalyzes the NAD-dependent conversion of L-leucine to α-ketoisocaproate. Suitable for the production of L-tertiary leucine amino acids. Has an optimal pH of 10.5-10.8 for L-leucine substrate and 9.4 for α-ketoisocaproate substrate. Has an optimal temperature of 65°C.

包装

100 u in Plastic ampoule

警告

Toxicity: Standard Handling (A)

单位定义

One unit is defined as the amount of enzyme that will convert 1.0 ?mol L-leucine to α-ketoisocaproate in the presence of NAD⁺per min at 30°C, pH 10.7.

外形

In 100 mM NaCl, 10 mM potassium phosphate buffer, 50% glycerol, pH 7.5.

重悬

Following initial thaw, aliquot and freeze (-20°C).

其他说明

K?rst, U., et al. 1986.FEMS Microbiol. Lett.37,335.
Schütte, H., et al. 1985.Appl. Microbiol.Biotechnol.22,306.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

基本信息

酶学委员会(EC)编号	1.4.1.9 ( BRENDA | IUBMB )
MDL编号	MFCD00147461

半岛bd体育手机客户端 性质

形式	liquid
质量水平	100
specific activity	≥10 units/mg protein ≥50 units/mL
manufacturer/tradename	Calbiochem?
储存条件	OK to freeze avoid repeated freeze/thaw cycles
运输	wet ice
储存温度	?20℃

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 2