Anti-U2AF⁶⁵antibody, Mouse monoclonal antibody produced in mouse

半岛bd体育手机客户端 说明

一般描述

Monoclonal Anti-U2AF⁶⁵(mouse IgG2b isotype) is derived from the MC3 hybridoma produced by the fusion of mouse myeloma cells (Ag8.653 cells) and splenocytes from BALB/c mice immunized with recombinant human U2AF⁶⁵. U1, U2, U4/U6, and U5 are small nuclear riboprotein (snRNPs) that are major subunits of spliceosomes. Spliceosomes also contain non snRNPs proteins such as alternative splicing factor/splicing factor 2 (ASF/SF2), 35 kDa spliceosomal component (SC-35) and U2 snRNP auxiliary splicing factor (U2AF). U2 snRNP auxiliary factor 65 kDa (U2AF⁶⁵) is a splicing factor which has three C-terminal RNA recognition motifs (RRMs) and an N-terminal arginine/serine-rich (RS) domain. This gene is mapped to human chromosome 19q13.42. Splicing factor U2AF⁶⁵kDa subunit is a non-snRNP protein component of spliceosome complexes.

免疫原

recombinant human U2AF⁶⁵.

应用

Monoclonal Anti-U2AF⁶⁵antibody produced in mouse has been used in:

• RNA immunoprecipitation
• immunoblotting
• chromatin immunoprecipitation (ChIP)
• enzyme-linked immunosorbent assay (ELISA)
• immunohistochemistry
• immunocytochemistry
• immunoelectron microscopy

生化/生理作用

U2 snRNP auxiliary factor 65 kDa (U2AF⁶⁵)/U2AF2 helps to supply the small nuclear ribonucleoprotein (snRNP) U2, which is subunit of the spliceosome. It can induce the exclusion of alternative exons. U2AF⁶⁵primarily regulates the splicing of pre-mRNA. The serine-rich (RS) repeats at the N-terminal region act as a signalling factor for nuclear localization. (U2AF⁶⁵) and (U2AF³⁵) are components of the splicing factor U2AF that transports between the cytoplasm and the nucleus.

外形

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号	MFCD03456068
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	200
生物来源	mouse
偶联物	unconjugated
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	MC3, monoclonal
形式	buffered aqueous solution
分子量	antigen ~65 kDa
species reactivity	human,Xenopus, rat, mouse
包装	antibody small pack of 25 μL
technique(s)	electron microscopy: suitable immunocytochemistry: suitable immunohistochemistry: suitable immunoprecipitation (IP): suitable indirect ELISA: suitable microarray: suitable western blot: 0.1-0.2 μg/mL using total cell extract from HeLa cells
同位素/亚型	IgG2b
UniProt登记号	P26368
运输	dry ice
储存温度	?20℃
Gene Information	human ... U2AF2(11338) mouse ... U2af2(22185) rat ... U2af2(308335)

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable