半岛bd体育手机客户端 说明

一般描述

Amicon^?Ultra 4 mL centrifugal devices with Ultracel^?3,000 Dalton molecular weight cutoff filters provide fast ultrafiltration, and reliable retention of macromolecules. For example, Cytochrome c (12.4 kDa) at 0.25 mg/ml initial concentration is retained at >95% .

The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 80-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss.

The concentrate is collected from the filter device sample reservoir using a pipetter, while the ultrafiltrate is collected in the provided centrifuge tube. The device can be spun in a swinging bucket or fixed angle rotor. With their vertical membrane design and deadstop, Amicon^?Ultra 15 mL filters deliver the highest recovery with short spin times.

应用

• Ultrafiltration with 3,000 Dalton molecular weight cutoff Ultracel^?membrane.
• Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms.
• Purification of macromolecular components found in tissue culture extracts or cell lysates and protein removal prior to HPLC.
• Concentration of dilute or pre-purified proteins from column eluents.
• Desalting, buffer exchange and protein dialysis.
• Size exclusion and protein fractionation.

特点和优势

• Ultracel^?regenerated cellulose membrane with 3 kDa NMWCO delivers retentate recovery of greater than 90%.
• Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 5 minutes).
• Heat-sealed membrane minimizes downstream extractables.
• 100% integrity tested for reliable performance.
• Convenient sample monitoring with translucent housing and volume gradations.
• Direct pipette sample access eliminates processing step to recover concentrate.
• High concentration factors of 80–100X.

外形

色码：透明，带有粉色盖

法律信息

Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany

基本信息

eCl@ss	36100101
NACRES	NB.24

半岛bd体育手机客户端 性质

质量水平	400
物料	polyethylene cap polyethylene liner polypropylene tube regenerated cellulose membrane regenerated cellulose support styrene-butadiene housing
无菌性	non-sterile
半岛bd体育手机客户端 线	Amicon?
manufacturer/tradename	Amicon?Ultra
technique(s)	protein extraction: suitable protein purification: suitable
长度	124 mm (4.88 in.)
直径	17.3 mm
过滤面积	3 cm2
孔径	3 kDa NMWCO
试验参数	sample volume: 4 mL
运输	ambient