Monoclonal Anti-Topoisomerase I antibody produced in mouse

半岛bd体育手机客户端 说明

一般描述

Monoclonal Anti-Topoisomerase I (mouse IgG1 isotype) is derived from the Mab1 hybridoma produced by the fusion of SP2/0 mouse myeloma cells and splenocytes from BALB/c mice immunized with human purified topoisomerase I. The protein family of DNA topoisomerases is divided into two types (type I and II). The type I enzymes include eukaryotic and bacterial topoisomerase I and III. Human DNA Topoisomerase I belong to the subtype IB enzymes. The protein has four distinct domains. There are four nuclear localization signals in the N-terminal domain. A linker domain of 77 amino acids follows the core domain. The fourth domain, which is 53 amino acids long, contains the active site Tyr⁷²³. Topoisomerase I (TOP1) gene is located on human chromosome 20q12.

免疫原

purified human topoisomerase I

应用

Monoclonal Anti-Topoisomerase I antibody produced in mouse has been used in western blot analysis.

包装

200 μL in glass bottle

生化/生理作用

DNA topoisomerases are enzymes that control the amount of supercoiling in DNA. Without topoisomerases, DNA cannot replicate normally. Type I enzymes of DNA topoisomerases introduce transient single strand breaks in DNA. Recently it was reported that DNA topoisomerase I is involved in modulation of RNA and is present in retroviral particles, like human immunodeficiency virus (HIV1). Furthermore, the enzyme enhances HIV-1 cDNA production in reverse transcription assaysin vitro. Topoisomerase I (TOP1) serves as swivel at the time of replication, transcription and recombination, which helps in releasing over winding of duplex DNA.

外形

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号	MFCD03097334
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	200
生物来源	mouse
偶联物	unconjugated
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	Mab1, monoclonal
形式	buffered aqueous solution
分子量	antigen ~100 kDa
species reactivity	human
technique(s)	microarray: suitable western blot: 1-2 μg/mL using A231 human kidney cells
同位素/亚型	IgG1
UniProt登记号	P11387
运输	dry ice
储存温度	?20℃
Gene Information	human ... TOP1(7150)