半岛bd体育手机客户端 说明
一般描述
TIP-associated protein (TAP), also known as nuclear export factor (NXF1), is encoded by the gene mapped to human chromosome 11q12.3. The encoded protein is a member of multigene family of evolutionarily conserved proteins. TAP contains non-canonical RNP-type RNA binding domain, four leucine-rich repeats and an NTF2-like domain. It also contains an ubiquitin-associated domain involved in the interaction with nucleoporins.
Monoclonal Anti-TAP (mouse IgG1 isotype) is derived from the 53H8 hybridoma produced by the fusion of mouse myeloma cells (SP2/O) and splenocytes from Balb/c mice immunized with TAP.
特异性
Monoclonal Anti-TAP recognizes human TAP (~66 kDa).
免疫原
TAP (Tip Associated Protein).
应用
Anti-TAP antibody, mouse monoclonal may be used in:
生化/生理作用
TIP-associated protein (TAP) plays an important role in carrying mRNAs from the nucleus. Elevated expression of TAP or of SRp20, enhance the production of gammaretroviral Gag polyprotein. The encoded protein might facilitate the repression of two mutations caused by insertions of endogenous retroviruses into introns of cellular genes by regulating their mature transcript levels ～2 fold.
Transporter associated with antigen processing (TAP) interacts with the RNA-binding protein Y14 protein, a participant of the mRNA-protein complex (mRNP) near the exon-exon junction of the spliced mRNAs. Formation of the TAP/p15 heterodimer is required for efficient nuclear pore complex binding and significantly enhances the ability of TAP to export mRNAs from the nucleus. In addition, TAP plays a central role in the nuclear export of (constitutive transport element) CTE containing viral mRNA. However, the recruitment of TAP to the two types of mRNA occurs by different mechanisms. TAP binds to the viral CTE directly, while the interaction with cellular mRNA is found to be indirectly.
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
储存及稳定性
For continuous use, store at 2-8 ℃ for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in “frost-free” freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| MDL编号 | MFCD01865352 |
| NACRES | NA.41 |
半岛bd体育手机客户端 性质
| 质量水平 | 200 |
| 生物来源 | mouse |
| 偶联物 | unconjugated |
| 抗体形式 | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| 克隆 | 53H8, monoclonal |
| 形式 | buffered aqueous solution |
| 分子量 | antigen ~66 kDa |
| species reactivity | human |
| technique(s) | immunocytochemistry: suitable immunoprecipitation (IP): suitable indirect ELISA: suitable microarray: suitable western blot: 0.5-1 μg/mL using HeLa nuclear cell extract |
| 同位素/亚型 | IgG1 |
| UniProt登记号 | Q9UBU9 |
| 运输 | dry ice |
| 储存温度 | ?20℃ |
| Gene Information | human ... NXF1(10482) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
