TrkB/Fc Chimera human

半岛bd体育手机客户端 说明

应用

TrkB/Fc Chimera human can be used in synaptoneurosome preparation and western blot analysis. Intracerebroventricular administeration of TrkB/Fc chimera human blocks TrkB ligand signaling and was used to study the role of BDNF in ATN response augmentation.

生化/生理作用

Member of the Trk family tyrosine kinase receptors. Binds specifically to mediate neurotrophins BDNF and NT-4/5.
TrkB/Fc Chimera human consist of extracellular domain of human TrkB1 fused to histidine-tagged Fc region of human IgG1 by using a polypeptide linker. TrkB belongs to tyrosine kinase receptors family and facilitates the hippocampal development as well as regulating the synaptic plasticity that underlies long-term potentiation and learning. Brain derived neurotrophic factor (BDNF) and Neurotrophin 4 or 5 are preferred ligands for TrkB. Nevertheless it can also bind to NTF3/neurotrophin-3 that is less efficient in activating the receptor but regulates neuron survival through NTRK2.

其他说明

Extracellular domain of human TrkB fused to the C-terminal histidine-tagged Fc region of human IgG1.

外形

Lyophilized from 0.2 μm filtered solution in phosphate buffered saline.

重悬

Reconstitute to 100 μg/mL in phosphate buffered saline.

分析说明

Inhibits BDNF-induced proliferation of TrkB transfected cells, BaF-TrkB-BD.

基本信息

MDL编号	MFCD03458837
NACRES	NA.32

半岛bd体育手机客户端 性质

质量水平	200
生物来源	human
重组	expressed in NSO cells
测定	>90% (SDS-PAGE)
形式	lyophilized powder
效能	0.1-0.4 μg/mL ED50
分子量	120-130 kDa by SDS-PAGE (reducing) calculated mol wt 71 kDa
包装	pkg of 100 μg
储存条件	avoid repeated freeze/thaw cycles
technique(s)	western blot: suitable
杂质	endotoxin, tested
UniProt登记号	Q16620
储存温度	?20℃
Gene Information	human ... NTRK2(4915)

安全信息

储存分类代码	13 - Non Combustible Solids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable