Tau-412 human

半岛bd体育手机客户端 说明

一般描述

Tau-412 human, (1N4R) recombinant, is one of the isoforms of Tau and lacks a projection domain region. The isoforms of tau are generated by alternate splicing. The N terminal region of tau is acidic and is separated from the C-terminal tubulin-binding region by a central proline-rich region. The isoforms differ in amino-terminal N1 and N2 regions as well as binding repeats at the tubulin region. Tau belongs to the family of neuronal microtubule-associated proteins.

生化/生理作用

Tau promotes assembly and stabilizes neuronal microtubules under normal physiological conditions. Under pathological conditions Tau can also undergo modifications such as hyperphosphorylation, which can result in the generation of aberrant aggregates such as found in neurofibrillary tangles in Alzheimer′s disease. Tau-412 human is purified without any acid treatment and is suitable for promoting microtubule assembly, and for hyperphosphorylation-induced self-assembly into filaments.
Isoform of Tau, variant 1N4R, having 4 microtubule binding repeats (R) and one amino terminal insert (N).

重悬

Lyophilized from MES, pH 6.8, containing NaCl and EGTA. When reconstituted in water to a protein concentration of 1 mg/mL, the resulting buffer will have ~50 mM MES, pH 6.8, 100 mM NaCl, and 0.5 mM EGTA.

基本信息

MDL编号	MFCD07785734
NACRES	NA.32

半岛bd体育手机客户端 性质

质量水平	200
生物来源	human
重组	expressed inE. coli
测定	≥90% (SDS-PAGE)
形式	lyophilized powder
分子量	42.9 kDa
UniProt登记号	P10636
application(s)	cell analysis
运输	dry ice
储存温度	?70℃
Gene Information	human ... MAPT(4137)

安全信息

储存分类代码	13 - Non Combustible Solids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable
个人防护装备	Eyeshields, Gloves, type N95 (US)