半岛bd体育手机客户端 说明
一般描述
Tumor necrosis factor (ligand) superfamily, member 11 (Tnfsf11) is also known as receptor activator of nuclear factor-κB ligand (RANKL). It is a type II transmembrane signaling receptor. It has a molecular weight of around 35kDa. This protein contains an amino-terminal intracellular tail and a carboxy-terminal extracellular region, that bears a connecting stalk and a receptor-binding domain. Tnfsf11 is located on human chromosome 13q14.11.
应用
RANK Ligand/TRANCE human has been used to stimulate differentiation.
RANK Ligand/TRANCE human can be used inin vitroosteoclastogenic assays for osteoclast differentiation.
生化/生理作用
RANK Ligand (receptor activator of NF-kB ligand (RANKL) also referred to as TNF-related activation induced cytokines (TRANCE) or osteoprotegerin ligand is a protein that belongs to tumor necrosis factor (TNF) family. It stimulates the mature dendritic cells and induces the cytokine production. RANKL also facilitates bone remodelling and possess an angiogenic activity.
RANK Ligand/TRANCE, a member of the TNF superfamily, induces activation of the c-jun N-terminal kinase, enhances T-cell growth and dendritic cell function, induces osteoclastogenesis, and lymph node organogenesis. Mouse and human RANK Ligand share 85% amino acid identity. RANK is the cell surface receptor for RANK Ligand.
外形
Lyophilized from a 0.2 μm filtered solution in 20 mM MOPS and 500 mM NaCl, pH 6.5, with 50 μg BSA per 1 μg as a carrier protein.
分析说明
The biological activity is measured by its ability to induce osteoclast differentiation on mouse splenocytes.
基本信息
MDL编号 | MFCD01867142 |
NACRES | NA.32 |
半岛bd体育手机客户端 性质
质量水平 | 200 |
生物来源 | human |
重组 | expressed in NSO cells |
测定 | >90% (SDS-PAGE) |
形式 | lyophilized powder |
效能 | 1.5-7.5 ng/mL ED50 |
分子量 | glycolysylated recombinant product ~35 kDa by SDS-PAGE calculated mol wt 23 kDa |
包装 | pkg of 10 μg |
储存条件 | avoid repeated freeze/thaw cycles |
杂质 | endotoxin, tested |
UniProt登记号 | O14788 |
application(s) | cell analysis |
储存温度 | ?20℃ |
Gene Information | human ... TNFSF11(8600) |
安全信息
储存分类代码 | 13 - Non Combustible Solids |
WGK | WGK 3 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
个人防护装备 | Eyeshields, Gloves, type N95 (US) |
Sigma-Aldrich