Tumor Necrosis Factor-β human

半岛bd体育手机客户端 说明

生化/生理作用

Tumor necrosis factor-β (TNF-β), also known as lymphotoxin-α (LT-α) and cytotoxic factor, is a 25 kDa glycoprotein that is tightly regulated by lymphocytes. TNF-β and TNF-α share a 28% amino acid sequence identity, and as soluble forms, both can exist in solution as homotrimers, activate the same TNF receptors (TNFR1 and TNFR2) and elicit many of the same bioactivities. Unlike TNF-α, TNF-β can also form heterotrimers with one or two molecules of the related membrane-bound lymphotoxin-β (LT-β). Reportedly, LTα1/β2, the predominant heterotrimer, binds and activates only the LT-β receptor, LT-βR. The other heterotrimer, LT-α2/β1 binds and activates TNFR1 and TNFR2 but not LT-βR. TNF-β is expressed in activated T, B and NK cells and in certain transformed cells. Genes for TNF-β, LT-β and TNF-α are tightly linked near each other within the MHC. TNF-β appears to play many of the same biological roles as TNF-α, but the membrane-bound form with LT-β is critical for normal lymphoid organ development. Genetic polymorphisms in TNF-α and TNF-β have been linked to certain pathological conditions, including myasthenia gravis. At the amino acid level, human and mouse TNF-β are 74% homologous and human and mouse LT-β are 80% homologous.

外形

lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein

分析说明

The biological activity is measured in a cytotoxicity assay using the TNF-suspectible murine L929 cell line in the presence of actinomycin D.

基本信息

MDL编号	MFCD00163958
NACRES	NA.77

半岛bd体育手机客户端 性质

质量水平	200
生物来源	human
重组	expressed inE. coli
测定	>95% (SDS-PAGE)
形式	powder
效能	4-20 pg/mL Activity
质量	endotoxin tested
分子量	protein 18.8 kDa
包装	pkg of 10 μg
储存条件	avoid repeated freeze/thaw cycles
technique(s)	cell culture | mammalian: suitable
杂质	≤1 EU/μg
UniProt登记号	P01374
储存温度	?20℃
Gene Information	human ... LTA(4049), TNFSF13B(10673)

安全信息

储存分类代码	13 - Non Combustible Solids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable
个人防护装备	Eyeshields, Gloves, type N95 (US)