半岛bd体育手机客户端 说明

一般描述

Thimet oligopeptidase is considered essential in the degradation of collagen in collaboration with collagenolytic enzymes. Thimet oligopeptidase (TOP) is a neuropeptidase involved in the hydrolysis of gonadotropin-releasing hormone, a key component of the hypothalamic-pituitary-gonadal axis.

应用

Thimet oligopeptidase can be used for the degradation of collagen in combination with collagenases. It can also be used for the hydrolysis of neuropeptides such as bradykin, neurotensin, and amyliod-β-peptide. Thimet oligopeptidase has been used in a study to investigate the effect of acute cocaine administration in male rats on TOP specific activity and mRNA levels in prosencephalic brain areas related with the reward circuitry: ventral striatum, hippocampus, and frontal cortex.

制备说明

This enzyme has been affinity chromatographically purified using a niquel affinity column. It contains a 6-Histidine tag in its C-terminus.

A working solution of this enzyme can be prepared in 20 mM phosphate buffered saline solution, pH 7.0, or sterile and deionized water, pH 7.0.

基本信息

酶学委员会(EC)编号	3.4.24.15 ( BRENDA | IUBMB )
NACRES	NA.54

半岛bd体育手机客户端 性质

质量水平	200
重组	expressed inE. coli
形式	powder
分子量	~77 kDa
储存温度	?20℃

安全信息

象形图
警示用语：	Warning
危险声明	H315 - H332
危险分类	Acute Tox. 4 Inhalation - Skin Irrit. 2
储存分类代码	13 - Non Combustible Solids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable