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Topoisomerase I human

品牌
Sigma-Aldrich
货号
T9069
规格纯度
buffered aqueous glycerol solution, ≥2 units/μL
价格
6027.95 *本价格含增值税费
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半岛bd体育手机客户端 名称:
Topoisomerase I human
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Topoisomerase I (TOP1) is mapped to human chromosome 20q12. TOP1 is a 91 kDa type IB enzyme and consists of N-terminal highly charged domain, a core, linker domain and a C-terminal catalytic domain with Tyr-723 active site residue.

应用

Topoisomerase I human has been used for Topoisomerase I assay for screening inhibitors.
Topoisomerase I has been used in a study to assess implications for the regulation of HIV-1 replication. Topoisomerase I has also been used in a study to investigate the tumor suppressor protein kinase Chk2 is a mediator of anoikis of intestinal epithelial cells.

生化/生理作用

Topoisomerase I human (TOP1) interacts with DNA by wrapping and takes a bi-lobed conformation. TOPI is essential for normal development and transcriptional regulation. Variants in the TOP1 gene is observed in patients with colorectal cancer.
Topoisomerase I relaxes supercoiled DNA molecules. The enzyme initiates transient breakages and rejoins of phosphodiester bonds in superhelical turns of closed-circular DNA. Enzyme activity is independent of right- and left-handed superhelices.

单位定义

One unit will relax 0.25 μg of supercoiled plasmid DNA in 30 minutes at pH 7.9 at 37 ℃.

外形

Solution containing 20 mM sodium phosphate, pH 7.4, 300 mM NaCl, 50 μg/mL BSA, 50% glycerol, and between 25-100 mM imidazole (concentration will be lot dependent).

基本信息

酶学委员会(EC)编号 5.99.1.2 ( BRENDA | IUBMB )
MDL编号 MFCD03458765
NACRES NA.54

半岛bd体育手机客户端 性质

质量水平 200
测定 ≥2 units/μL
形式 buffered aqueous glycerol solution
分子量 100 kDa
浓度 ≥2 units/μL
UniProt登记号 P11387
储存温度 ?70℃
Gene Information human ... TOP1(7150)

安全信息

象形图 GHS08
警示用语: Danger
危险声明 H360D
预防措施声明 P201 - P308 + P313
危险分类 Repr. 1B
储存分类代码 6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
WGK WGK 1
闪点(F) Not applicable
闪点(C) Not applicable

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