Topoisomerase I human

半岛bd体育手机客户端 说明

一般描述

Topoisomerase I (TOP1) is mapped to human chromosome 20q12. TOP1 is a 91 kDa type IB enzyme and consists of N-terminal highly charged domain, a core, linker domain and a C-terminal catalytic domain with Tyr-723 active site residue.

应用

Topoisomerase I human has been used for Topoisomerase I assay for screening inhibitors.
Topoisomerase I has been used in a study to assess implications for the regulation of HIV-1 replication. Topoisomerase I has also been used in a study to investigate the tumor suppressor protein kinase Chk2 is a mediator of anoikis of intestinal epithelial cells.

生化/生理作用

Topoisomerase I human (TOP1) interacts with DNA by wrapping and takes a bi-lobed conformation. TOPI is essential for normal development and transcriptional regulation. Variants in the TOP1 gene is observed in patients with colorectal cancer.
Topoisomerase I relaxes supercoiled DNA molecules. The enzyme initiates transient breakages and rejoins of phosphodiester bonds in superhelical turns of closed-circular DNA. Enzyme activity is independent of right- and left-handed superhelices.

单位定义

One unit will relax 0.25 μg of supercoiled plasmid DNA in 30 minutes at pH 7.9 at 37 ℃.

外形

Solution containing 20 mM sodium phosphate, pH 7.4, 300 mM NaCl, 50 μg/mL BSA, 50% glycerol, and between 25-100 mM imidazole (concentration will be lot dependent).

基本信息

酶学委员会(EC)编号	5.99.1.2 ( BRENDA | IUBMB )
MDL编号	MFCD03458765
NACRES	NA.54

半岛bd体育手机客户端 性质

质量水平	200
测定	≥2 units/μL
形式	buffered aqueous glycerol solution
分子量	100 kDa
浓度	≥2 units/μL
UniProt登记号	P11387
储存温度	?70℃
Gene Information	human ... TOP1(7150)

安全信息

象形图
警示用语：	Danger
危险声明	H360D
预防措施声明	P201 - P308 + P313
危险分类	Repr. 1B
储存分类代码	6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable