半岛bd体育手机客户端 说明

一般描述

The trypsin molecule has two domains: one is related to the enzyme active site and the tryptophan residues; the other is related to the 8-anilinonaphthalene-1-sulfonate binding.

应用

An experimental approach using SDS-PAGE, in-gel trypsin digestion and proteomic analysis identified five endosomal proteins. Trypsin cleavage of the high-risk HPV-16 E5 protein (16E5) generates a unique four-amino acid C-terminal peptide that serves as a marker for E5 expression in transfected cells and epithelial cell lines containing integrated and episomal HPV-16 DNA.

单位定义

One unit will hydrolyze 1.0 μmole of BAEE per min at pH 8.0 at 30 ℃ (titrimetric assay).

外形

Suspension in approx. 10 mM acetic acid, pH 3.2, containing preservative

制备说明

TPCK-treated

基本信息

MDL编号	MFCD00082094
eCl@ss	42020142
NACRES	NA.54

半岛bd体育手机客户端 性质

质量水平	200
生物来源	bovine pancreas (trypsin)
形式	buffered aqueous suspension
浓度	≥15 units/mL (packed gel)
标记范围	≥15 units per mL (packed gel)
基质	beaded agarose
储存温度	2-8℃

安全信息

象形图
警示用语：	Warning
危险声明	H317
预防措施声明	P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
危险分类	Skin Sens. 1
储存分类代码	10 - Combustible liquids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable