Stromal Cell-Derived Factor 1β/pre-B Cell Growth Stimulating Factor human

半岛bd体育手机客户端 说明

一般描述

C-X-C motif chemokine ligand 12 (CXCL12), also known as stromal cell-derived factor 1 α (SDF-1a), is encoded by the gene mapped to human chromosome 10q11. Stromal cell-derived factor 1 α and β (SDF-1 α and β) are stromal derived CXC chemokines and signal through the C-X-C motif chemokine receptor-4 (CXCR4). CXCL12 is mainly expressed in the inflamed and failing myocardium.

应用

Stromal Cell-Derived Factor 1β has been used to activate chemokine receptor-4 (CXCR4) and to further assess its role in tumor cell migration using chemotaxis assay.

生化/生理作用

SDF-1/PBSF is a chemoattractant for T lymphocytes and monocytes, but not neutrophils. It is highly conserved between species, with only one amino acid substitution between the human and mouse proteins.
C-X-C motif chemokine ligand 12 (CXCL12) is involved in inflammation, proliferation, tumorigenicity and metastasis. CXCL12 exerts HIV (human immunodeficiency virus) suppressive activity in cells expressing the CXCR4 (C-X-C motif chemokine receptor 4). It is overexpressed in breast carcinoma, ovarian carcinoma and papillary thyroid carcinoma. It enhances angiogenesis in the ischemic tissue. SDF1 is also implicated in neuroprotection after ischemic stroke with the help of CXCR4.
SDF-1/PBSF, a member of the α (CXC) chemokine family, is a chemoattractant for T lymphocytes and monocytes, but not neutrophils. SDF-1α and SDF-1β (together also known as SDF-1/PBSF) are encoded by a single gene and arise by alternative splicing. The two proteins are identical except for the four amino acid residues that are present in the C-terminal of SDF-1β and absent from SDF-1α. SDF-1/PBSF is highly conserved between species, with only one amino acid substitution between the human and mouse proteins.

外形

Lyophilized from 30% acetonitrile and 0.1% trifluoracetic acid containing 0.5 mg of bovine serum albumin.

分析说明

The biological activity is measured by its ability to chemoattract 8-10 day human T lymphocytes cultured in the presence of IL-2.

基本信息

MDL编号	MFCD01094859
NACRES	NA.32

半岛bd体育手机客户端 性质

质量水平	100
生物来源	human
重组	expressed inE. coli
测定	>97% (SDS-PAGE)
形式	lyophilized powder
效能	10-30 ng/mL ED50
分子量	~8 kDa
包装	pkg of 10 μg
储存条件	avoid repeated freeze/thaw cycles
technique(s)	cell culture | mammalian: suitable
杂质	endotoxin, tested
UniProt登记号	P48061
储存温度	?20℃
Gene Information	human ... CXCL12(6387)

安全信息

储存分类代码	11 - Combustible Solids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable