Stromal Cell-Derived Factor 1α/pre-B Cell Growth Stimulating Factor from mouse

半岛bd体育手机客户端 说明

一般描述

Stromal derived factor-1 (SDF-1) is localized to adipocytes.

应用

Stromal Cell-Derived Factor 1α/pre-B Cell Growth Stimulating Factor from mouse has been used in invasion assay. It has also been used to study the SDF-1 release from chitosan-based nanoparticles.

生化/生理作用

Stromal derived factor-1 (SDF-1) is an insulin-desensitizing factor, upregulated during both starvation and obesity. Exogenic SDF-1 reduces insulin-mediated signaling and glucose uptake. SDF-1 mediates migration of cortical interneurons and endothelial progenitor cells of the bone marrow through chemotaxis.
SDF-1α/PBSF, a member of the α (CXC) chemokine family, is a chemoattractant for T lymphocytes and monocytes, but not neutrophils. SDF-1α/PBSF is highly conserved between species, with only one amino acid substitution between the human and mouse proteins. SDF-1α and SDF-1β (together also known as SDF-1α/PBSF) are encoded by a single gene and arise by alternative splicing. The two proteins are identical except for the four amino acid residues that are present in the C-terminal of SDF-1β and absent from SDF-1α..

外形

Lyophilized from a 0.2 μm filtered solution in 30% acetonitrile and 0.1% trifluoroacetic acid containing 0.5 mg bovine serum albumin.

制备说明

Recombinant Mouse Stromal Cell-Derived Factor 1α (SDF-1α)/Pre-B Cell Growth Stimulating Factor (PBSF) is produced from a DNA sequence encoding the mature mouse SDF-1α protein sequence (amino acid residues 22-89) expressed inEscherichia coli.Recombinant mouse SDF-1α (68 amino acids) has a predicted molecular mass of approximately 8 kDa.

分析说明

The biological activity is measured by its ability to chemoattract cultured human lymphocytes and mouse BaF3 cells transfected with human CXCR4.

基本信息

MDL编号

MFCD02100631

半岛bd体育手机客户端 性质

质量水平	200
生物来源	mouse
重组	expressed inE. coli
测定	>97% (SDS-PAGE)
形式	powder
效能	0.15-0.6 ng/mL ED50
质量	endotoxin tested
分子量	predicted mol wt ~8 kDa
包装	pkg of 10 μg
储存条件	avoid repeated freeze/thaw cycles
technique(s)	cell culture | mammalian: suitable
杂质	<1 EU/μgtested (LAL test)
UniProt登记号	P40224
储存温度	?20℃
Gene Information	mouse ... Cxcl12(20315)

安全信息

储存分类代码	13 - Non Combustible Solids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable
个人防护装备	Eyeshields, Gloves, type N95 (US)