Anti-trimethyl Histone H3 (Lys79) Antibody, Trial Size

半岛bd体育手机客户端 说明

特异性

This antibody recognizes Histone H3 methylated at Lys79.
Broad species reactivity is expected.

免疫原

Epitope: Lys79
Linear Peptide corresponding to human Histone H3 methylated at Lys79.

应用

Dot Blot Specificity Analysis:
AbSurance Histone H3 Antibody Specificity Arrays (Cat. #: 16-667 and 16-665) containing peptides to all key histone modification sites, were probed with Anti-trimethyl Histone H3 (Lys79) Antibody Antibody ; dilution 1:5000. Proteins were visualized using a goat anti-mouse IgG (H+L) conjugated to HRP and a chemiluminescence detection system.


Multiplex Immunoassay
Specificity of interaction of anti-trimethyl histone H3 Lys79 antibody was assayed using the Luminex multiplex immunoassay platform. Anti-trimethyl-Histone H3 (Lys79) diluted to 0.01 mg/ml concentration was incubated with a cocktail of xMAP microspheres conjugated to the following histone H3 peptides, monomethyl Lys79, unmodified Lys79, trimethyl Lys 27, trimethyl Lys 9, trimethyl Lys79 processed and detected using standard xMAP protocols.
Anti-trimethyl Histone H3 (Lys79) Antibody is a rabbit polyclonal antibody for detection of trimethyl Histone H3 (Lys79) also known as H3K79me2, Histone H3 (di methyl K79) & has been validated in WB, Mplex.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones

Chromatin Biology

质量

Evaluated by Western Blot in HeLa acid extract lysate.

Western Blot Analysis: 0.1 ?g/ml of this antibody detected Histone H3 on 10 ?g of HeLa acid extract lysate.

目标描述

~ 17 kDa

外形

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Affinity purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
HeLa acid extract lysate and recombinant Histone H3

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	100
抗体形式	affinity isolated antibody
antibody product type	primary antibodies
克隆	polyclonal
纯化方式	affinity chromatography
species reactivity	human
technique(s)	dot blot: suitable multiplexing: suitable western blot: suitable
NCBI登记号	NP_003484.1
UniProt登记号	Q16695
运输	wet ice

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable