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位置: 首页> 品牌> Sigma-Aldrich> Anti-monomethyl Histone H4 (Lys20) Antibody, Trial Size

Anti-monomethyl Histone H4 (Lys20) Antibody, Trial Size

品牌
Sigma-Aldrich
货号
07-1570-S
规格纯度
Upstate?, from rabbit
价格
1660.9 *本价格含增值税费
促销
服务
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  • 增值税票
数量
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半岛bd体育手机客户端 名称:
Anti-monomethyl Histone H4 (Lys20) Antibody, Trial Size
H4K20me1, Histone H4 (monomethyl K20), H4 histone family, member A, Histone 1, H4a, Histone cluster 1, H4a
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. Histones modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. These modifications can alter local chromatin architecture, or recruit trans-acting factors that recognize specific histone modifications (the "histone code" hypothesis). Methylation of histone H4 at lysine 20 has been implicated in transcriptional activation, gene silencing, heterochromatin formation, mitosis, and DNA repair. Histone H4 is progressively methylated at Lys20 during the G(2), M, and G(1) phases of the cell cycle, and methylation of H4Lys20 is a marker for heterochromatin. In mammals, the female X chromosome is coated by the Xist RNA, which is critical for silencing, and is marked mainly by methylation of H3Lys9, H3Lys27 and H4Lys20.

特异性

Based on sequence homology, broad species cross-reactivity is expected
Recognizes monomethylated Histone H4
(Lys20), Mr 11 kDa.

免疫原

Epitope: Monomethyl Lys20
KLH-conjugated, linear synthetic peptide corresponding to amino acids 15-24
(AKRHR[me1K]VLRD-C) of human Histone H4.

应用

Anti-monomethyl Histone H4 (Lys20) Antibody, Trial Size is a rabbit polyclonal antibody for detection of monomethyl Histone H4 (Lys20) also known as H4K20me1, Histone H4 (mono methyl K20), H4 histone family member A & has been validated in WB.
Research Sub Category
Chromatin Biology

Histones
Chromatin Immunoprecipitation:
Sonicated chromatin prepared from HeLa cells (2 X 106cell equivalents per IP) was subjected to chromatin immunoprecipitation using 4 μg of either a negative control antibody or Anti-Monomethyl-Histone H4
(Lys20) antibody and the Magna ChIP A Kit (Cat. #17-610). Successful immunoprecipitation of monomethyl-histone H4 (Lys20)-associated DNA fragments was verified by qPCR using GAPDH coding region ChIP Primers versus Control Primers corresponding to the GAPDH promoter.
Please refer to the EZ-Magna ChIP A (Cat. # 17-408) or EZ-ChIP (Cat. # 17-371) protocol for experimental details.

Dot Blot Analysis : Absurance Histone H3 Antibody Specificity Array (Cat. No. 16-667) and Absurance Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665), which contain histone peptides with various modifications were probed with Cat. No. 07-1570-S Anti-monomethyl Histone H4 (Lys20) at 1:1000 dilution. Proteins were visualized using a Donkey anti-rabbit IgG conjugated to HRP and a chemiluminescence detection system.

Western Blot Analysis:
0.1 μg/mL of proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system (Please see figures)

Peptide Blocking Assay:
40 μg of histone H4 peptide containing monomethyl Lys20 abolished detection of histone H4 by anti-monomethyl-Histone H4 (Lys20) in immunoblot analysis of acid extracts of HeLa cells (Please see figures).
Research Category
Epigenetics & Nuclear Function

质量

Routinely evaluated by western blot analysis

目标描述

~11 kDa

外形

Purified from serum by affinity chromatography. Supplied at 1 mg/ml in 0.1M Tris-Glycine (pH7.4), 15mM NaCl, 0.05% NaN3.
Affinity Chromatography

储存及稳定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

分析说明

Control
HeLa cell acid extracts.

法律信息

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss 32160702
NACRES NA.41

半岛bd体育手机客户端 性质

生物来源 rabbit
质量水平 100
抗体形式 affinity isolated antibody
antibody product type primary antibodies
克隆 polyclonal
纯化方式 affinity chromatography
species reactivity human
manufacturer/tradename Upstate?
technique(s) ChIP: suitable
dot blot: suitable
inhibition assay: suitable (peptide)
western blot: suitable
NCBI登记号 NM_003538.3
UniProt登记号 P62805
运输 wet ice

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK WGK 1
闪点(F) Not applicable
闪点(C) Not applicable

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