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位置: 首页> 品牌> Sigma-Aldrich> Anti-Histone H3 Antibody, (Unmodified Lys4), clone CMA301, Trial Size

Anti-Histone H3 Antibody, (Unmodified Lys4), clone CMA301, Trial Size

品牌
Sigma-Aldrich
货号
05-1341-S
规格纯度
clone CMA301, from mouse, purified by affinity chromatography
价格
1726.4 *本价格含增值税费
促销
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数量
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半岛bd体育手机客户端 名称:
Anti-Histone H3 Antibody, (Unmodified Lys4), clone CMA301, Trial Size
H3 histone family, member T, Histone , , H3, Histone cluster 3, H3
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. Histone proteins are highly post-translationally modified, and Histone H3 is the most extensively modified of the five histones. The term "Histone H3" alone is purposely ambiguous in that it does not distinguish between sequence variants or modification state. Histone H3 is an important protein in the emerging field of epigenetics, where its sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes.

特异性

This antibody recognizes Histone H3 that is unmodified at Thr3 and Lys4 but does not recognize phosphorylated Thr3 nor acetylated or methylated Lys4. The antibody binding specificity allows modifications of Lys9, Ser10, and/or Lys141.
Broad species cross-reactivity is expected, based on sequence homology.

免疫原

Synthetic peptide corresponding to amino acids 1-12 of human Histone H3, conjugated to KLH.
Epitope: N-terminus (unmodified)

应用

Use Anti-Histone H3 Antibody, (Unmodified Lys4), clone CMA301, Trial Size (Mouse Monoclonal Antibody) validated in WB, ICC, ELISA, IP, FC, DB to detect Histone H3 also known as histone 3-H3.
ELISA:
This antibody has been shown by an outside laboratory to be suitable for ELISA.1

Immunocytochemistry:
This antibody has been shown by an outside laboratory to be suitable for immunocytochemistry.1

Immunoprecipitation:
This antibody has been shown by an outside laboratory to be suitable for immunoprecipitation.1
Research Sub Category
Histones
Research Category
Epigenetics & Nuclear Function

质量

Routinely evaluated by Western Blot on HeLa cell lysate.

Western Blot Analysis: 1:10,000 dilution of this antibody detected histone H3 in 10 μg of HeLa Acid Extract cell lysate and recombinant histone H3 (500 ng) but not other recombinant histones.

目标描述

Approx. 17 kDa

外形

Mouse monoclonal IgG2bκ supernatant in PBS with 0.05% sodium azide.
Peptide affinity chromatography

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

分析说明

Control
HeLa Acid extract lysate.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss 32160702
NACRES NA.41

半岛bd体育手机客户端 性质

质量水平 100
生物来源 mouse
抗体形式 affinity purified immunoglobulin
antibody product type primary antibodies
克隆 CMA301, monoclonal
纯化方式 affinity chromatography
species reactivity human, vertebrates
technique(s) ELISA: suitable
dot blot: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
同位素/亚型 IgG2bκ
NCBI登记号 NM_003493
UniProt登记号 Q16695
运输 wet ice

安全信息

储存分类代码 10 - Combustible liquids
WGK WGK 2
闪点(F) Not applicable
闪点(C) Not applicable

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