半岛bd体育手机客户端 说明
一般描述
Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming the nucleosome. Histones are modified post-translationally; and these modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. Phosphorylation at Ser10, which is linked to gene activation, prevents methylation at Lys9 but facilitates acetylation of H3 and H4. Phosphorylated Ser10 is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition, Ser10 phosphorylation is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at Ser10 by Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin.
特异性
This antibody specifically recognizes Histone H3 phosphorylated at Ser10.
Broad species cross-reactivity is expected, based on sequence homology.
免疫原
Epitope: Phospho Ser10
Synthetic peptide corresponding to amino acids 1-19 of human Histone H3, phosphorylated on Ser10, conjugated to KLH.
应用
Research Sub Category
Chromatin Biology
Histones
Research Category
Epigenetics & Nuclear Function
Anti-phospho-Histone H3 (Ser10) Antibody, clone CMA312 is a mouse monoclonal antibody for detection of phospho-Histone H3 (Ser10) also known as H3S10P, Histone H3 (phospho S10) & has been validated in WB, ELISA, ICC, Multiplexing and ChIP.
质量
Western Blot Analysis: 1 μg/mL of this antibody detected phospho-Histone H3 (Ser10) on 1 μg of colcemid-treated HeLa acid extract.
目标描述
~17 kDa
外形
Protein G Chromatography
Format: Purified
Purified mouse monoclonal IgG in PBS with 0.05% sodium azide.
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at 2-8°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
分析说明
Control
HeLa Acid extract lysate
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
半岛bd体育手机客户端 性质
| 质量水平 | 100 |
| 生物来源 | mouse |
| 抗体形式 | purified antibody |
| antibody product type | primary antibodies |
| 克隆 | CMA312, monoclonal |
| species reactivity | vertebrates, human |
| manufacturer/tradename | Upstate? |
| technique(s) | ChIP: suitable ELISA: suitable immunocytochemistry: suitable multiplexing: suitable western blot: suitable |
| 同位素/亚型 | IgG1 |
| NCBI登记号 | NP_003484.1 |
| UniProt登记号 | P84243 |
| 运输 | wet ice |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 2 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
