Anti-trimethyl Histone H3 (Lys36) Antibody, Trial Size

半岛bd体育手机客户端 说明

一般描述

Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming a nucleosome, the basic subunit of chromatin. Histones are modified post-translationally by the actions of enzymes in both the nucleus and cytoplasm. These modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. These modifications can alter local chromatin architecture, or recruit trans-acting factors that recognize specific histone modifications (the "histone code" hypothesis). The modifications occur predominantly on the N-terminal and C-terminal tails that extend beyond the nucleosome core particle. Methylation of histone H3 on Lys36 (H3K36me2/3) is tightly associated with actively transcribed genes, and this modification is found primarily within the coding region, suggesting H3K36 methylation is necessary for efficient RNA polymerase II elongation and processivity.

特异性

This antibody recognizes Histone H3 trimethylated at Lys36.

免疫原

Linear peptide corresponding to human Histone H3 trimethylated at Lys36.
Epitope: Trimethylated Lys36

应用

Use Anti-trimethyl Histone H3 (Lys36) Antibody (rabbit polyclonal antibody), Trial Size/ Pack validated in WB, DB, ICC, ChIP to detect trimethyl Histone H3 (Lys36) also known as H3K36me3, Histone H3 (tri methyl K36).
Research Sub Category
Histones
Research Category
Epigenetics & Nuclear Function
Dot Blot Analysis: A 1:500 dilution from a representative lot detected trimethyl Histone H3 (Lys36).

Chromatin Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in mouse embryonic stem cells. (Laboratory of Dr. Rob Klose, Oxford University)

Immunofluorescence Analysis: A representative lot was used by an independent laboratory in NIH/3T3 fibroblasts. (Laboratory of Dr. Rob Klose, Oxford University)

质量

Evaluated by Western Blot in Histone H3 recombinant proteins.

Western Blot Analysis: A 1:2,000 dilution of this antibody detected Histone H3 on 10 ?g of Histone H3 recombinant proteins.

目标描述

~17 kDa observed

外形

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4) and 150 mM NaCl with 0.05% sodium azide.
Affinity purified

储存及稳定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

分析说明

Control
Histone H3 recombinant proteins

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	100
抗体形式	affinity isolated antibody
antibody product type	primary antibodies
克隆	polyclonal
纯化方式	affinity chromatography
species reactivity	mouse, human
technique(s)	ChIP: suitable dot blot: suitable immunocytochemistry: suitable western blot: suitable
NCBI登记号	NP_003520
UniProt登记号	P68431
运输	wet ice
Gene Information	human ... HIST1H3F(8968)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable