半岛bd体育手机客户端 说明
一般描述
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
特异性
This antibody recognizes Histone H3 methylated at Lys9.
Broad species cross-reactivity is expected based on sequence homology.
免疫原
Epitope: Lys9
KLH-conjugated branched peptide corresponding to human Histone H3 methylated at Lys9.
应用
Anti-monomethyl-Histone H3 (Lys9) Antibody, Trial Size is a rabbit polyclonal for detection of Histone H3 monomethylated on lysine 9. Also known as H3K9me1, this antibody has dot blot proven specificity, and has been validated in WB, ICC, PIA. Small pack size is ideal for screening.
Research Sub Category
Histones
Research Category
Epigenetics & Nuclear Function
Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected Histone H3 in HeLa and A431 cells.
Peptide Inhibition Analysis: 1:10,000 dilution from a representative lot was used with HeLa acid extract for peptide block analysis.
质量
Evaluated by Western Blot in HeLa acid extract lysate.
Western Blot Analysis: 0.1 ?g/ml of this antibody detected Histone H3 on 10 ?g of HeLa acid extract lysate.
目标描述
~ 17 kDa
联系
Replaces: 07-395
外形
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Affinity purified
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
分析说明
Control
HeLa acid extract lysate
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
半岛bd体育手机客户端 性质
生物来源 | rabbit |
质量水平 | 100 |
抗体形式 | purified antibody |
antibody product type | primary antibodies |
克隆 | polyclonal |
纯化方式 | affinity chromatography |
species reactivity | human |
technique(s) | dot blot: suitable immunocytochemistry: suitable inhibition assay: suitable (peptide) western blot: suitable |
NCBI登记号 | NP_003484.1 |
UniProt登记号 | Q16695 |
运输 | wet ice |
Gene Information | human ... HIST1H3F(8968) |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich