Anti-Caspase3 (active form) Antibody, clone 3D9.3

半岛bd体育手机客户端 说明

一般描述

Caspase-3 (CPP32, Apopain) is the most extensively studied apoptotic protein. Caspase-3 is synthesized as an inactive proenzyme (32 kDa) that is processed in cells undergoing apoptosis by self-proteolysis and/or cleavage by another upstream protease. The processed form of caspase-3 consists of large (17 kDa) and small (12 kDa) subunits which associate to form an active enzyme. The active caspase-3 proteolytically cleaves and activates other caspases, as well as relevant targets in the cells such as PARP and DFF.

特异性

This antibody demonstrates specificity for the cleaved 17 kDa active form, but does not cross-react with the non-active (pro) form Caspase3 at 35 kDa.

免疫原

KLH-conjugated linear peptide corresponding to human Caspase3 (active form).

应用

Anti-Caspase3 (active form), clone 3D9.3 is an antibody targeting the Caspase3 (active form) protein, validated for use in WB, ICC & Flow.
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Caspase3 (active form) in Hela and Hela/staurosporine 3 hr cells.
Flow Cytometry Analysis: A 1:400 dilution from a representative lot detected Caspase3 (active form) in Jurkat T cells.

质量

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.1 ?g/mL of this antibody detected Caspase3 (active form) in 10 ?g of HeLa and staurosporine treated HeLa cell lysate.

目标描述

~17 kDa observed. Caspase3 is cleaved during apoptosis into 17 kDa and 12 kDa active forms.

联系

Replaces: 04-1090; 04-439

外形

Purified by Ion-Exchange Chromatography
Format: Purified

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	3D9.3, monoclonal
species reactivity	human
technique(s)	flow cytometry: suitable immunocytochemistry: suitable western blot: suitable
同位素/亚型	IgMκ
NCBI登记号	NP_004337
UniProt登记号	P42574
运输	wet ice
Gene Information	human ... CASP3(836)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable