Anti-Caspase 3 Antibody

半岛bd体育手机客户端 说明

一般描述

Caspase-3 (EC 3.4.22.56; UniProt P42574; also known as Apopain, CASP-3, CPP-32, PARP cleavage protease, SCA-1, SREBP cleavage activity 1, Caspase 3 apoptosis-related cysteine protease, Cysteine protease CPP32, Procaspase3, Protein Yama) is encoded by the CASP3 (also known as CPP32, CPP32B) gene (Gene ID 836) in human. Cysteine-aspartic proteases or Caspases play essential roles in apoptosis, necrosis, and inflammation. Historically, caspases were numbered in the order in which they were identified. Caspase-3 is initially produced with a propeptide sequence (a.a. 1-9), the removal of which yields the 268 a.a. caspase-3 proenzyme. Upon activation, the proenzyme is proteolytically cleaved first between Asp175-Ser176 to generate a p20 (a.a. 10-175) fragment and the p12 (a.a. 176-277) subunit. Further cleavage of the p20 fragment between Asp28-Ser29 produces the p17 (a.a. 29-175) subunit. The p17 and p12 dimerizes and forms the active caspase-3 enzyme. Caspase-3 plays a central role in the sequential activation of caspases during the execution phase of cell apoptosis. Caspase-3 mediates the proteolytic activation of caspases-6 and -7, while caspase-3 itself is processed and activated by caspase-8, -9, or -10. Caspase-3 is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer′s disease.

特异性

Expected to react with pro-caspase 3, the uncleaved/inactive caspase 3 (32 kDa), and the p12 (12 kDa), but not the p17 (17 kDa) subunit of cleaved/active caspase 3.

免疫原

KLH-conjugated linear peptide corresponding to human Caspase 3 near the C-terminus.
Epitope: Near C-terminus

应用

Anti-Caspase 3 Antibody is an antibody against Caspase 3 Antibody for use in Western Blotting and Immunocytochemistry.
Research Sub Category
Apoptosis - Additional
Immunocytochemistry Analysis: 4.0 ?g/mL from a representative lot detected Caspase 3 in HeLa, A431, HUVEC, and NIH/3T3 cells.
Research Category
Apoptosis & Cancer

质量

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: 0.05 ?g/mL of this antibody detected Caspase 3 in 10 ?g of A431 cell lysate.

目标描述

~32 kDa observed

联系

Replaces: 06-375

外形

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	100
抗体形式	affinity isolated antibody
antibody product type	primary antibodies
克隆	polyclonal
纯化方式	affinity chromatography
species reactivity	mouse, human
species reactivity (predicted by homology)	canine (based on 100% sequence homology), hamster (based on 100% sequence homology), nonhuman primates (based on 100% sequence homology), feline (based on 100% sequence homology), rat (based on 100% sequence homology)
technique(s)	immunocytochemistry: suitable western blot: suitable
NCBI登记号	NP_004337
UniProt登记号	P42574
运输	wet ice
Gene Information	human ... CASP3(836)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable