Anti-Tau Antibody, phosphoThreonine 231, clone PHF-6

半岛bd体育手机客户端 说明

特异性

Reacts with human Tau phosphorylated at threonine 231 and fetal tau. The antibody also reacts with dephosphorylated neurofibrillary tangles. MAB5450 is reactive with the Thr231 phosphorylated and diphosphorylated peptides. No reactivity with normal adult tau or with unphosphorylated or serine 235 phosphorylated protein.

免疫原

Epitope: phosphoThreonine 231
Paired helical filaments tau preparation from human brain.

应用

Western blot: 1:1000, non-phosphate buffers recommended. Specific for phospho-tau however highly phosphorylated blocking materials like non-fat milk can sometimes cause difficulties, thus we generally recommend blocking western blots with TBS-1-2% BSA solutions (filtered through a 0.45μm membrane) for better results.

Immunohistochemistry: fresh frozen tissues with Tris-NaCl-Triton treatment

{http://www.jhc.org/cgi/content/full/48/12/1627} & http://ajp.amjpathol.org/cgi/content/full/160/6/2045

Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Anti-Tau Antibody, phosphoThreonine 231, clone PHF-6 is an antibody against Tau for use in ELISA & WB.
Research Sub Category
Neurodegenerative Diseases

外形

Liquid.

储存及稳定性

Maintain at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	ascites fluid
克隆	PHF-6, monoclonal
species reactivity	human
manufacturer/tradename	Chemicon?
technique(s)	ELISA: suitable western blot: suitable
同位素/亚型	IgG1
NCBI登记号	NM_005910.3 NM_016834.2 NM_016835.2 NM_016841.2
UniProt登记号	P10636
运输	dry ice

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable