Anti-Calponin-1 Antibody

半岛bd体育手机客户端 说明

一般描述

Calponin 1 is a 34 kDa protein that is predominantly found in smooth muscle tissue. It is a high-affinity actin-binding protein, but also interacts with tropomyosin, and calmodulin to modulate muscle contraction. When bound to F-actin, it inhibits the activity of the actin-activated myosin ATPase. Calponin 1 is regulated by reversible phosphorylation involving PKC and CAMKII kinases, and type 2A phosphatases. In non-muscle cells, calponin 1 may play a role in cell migration, motility, and proliferation.

特异性

This antibody recognizes the C-terminus of Calponin-1.
Other homologies: Rat (92% sequence homology) and Mouse (83% sequence homology).

免疫原

KLH-conjugated linear peptide corresponding to the C-terminus of human Calponin-1.
Epitope: C-terminus

应用

Research Category
Cell Structure
Immunohistochemistry Analysis: A 1:1,000 dilution from a reprsesentative lot detected Calponin-1 in human seminal vesicle tissue.

Immunofluorescence Analysis: A 1:500 dilution from a representative lot detected Calponin-1 in smooth muscle cells from human seminal vesicle tissue.
This Anti-Calponin-1 Antibody is validated for use in Western Blotting, IHC(P), Immunofluorescence for the detection of Calponin-1.
Research Sub Category
Cytoskeleton

质量

Evaluated by Western Blot in human aortic smooth muscle tissue.

Western Blot Analysis: 0.1 ?g/mL of this antibody detected Calponin-1 in human aortic smooth muscle tissue.

目标描述

~33 kDa observed

联系

Replaces: 04-589

外形

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
Human aortic smooth muscle tissue

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	100
抗体形式	affinity isolated antibody
antibody product type	primary antibodies
克隆	polyclonal
纯化方式	affinity chromatography
species reactivity	human
species reactivity (predicted by homology)	chimpanzee (based on 100% sequence homology), sheep (based on 100% sequence homology), bovine (based on 100% sequence homology)
technique(s)	immunofluorescence: suitable immunohistochemistry: suitable (paraffin) western blot: suitable
NCBI登记号	NP_001290
UniProt登记号	P51911
运输	wet ice
Gene Information	human ... CNN1(1264)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable