半岛bd体育手机客户端 说明
一般描述
Rabbit polyclonal antibody supplied as lyophilized, undiluted serum. Recognizes the ~50 kDa dopamine D1receptor protein.
Recognizes the dopamine D1receptor in rat brain. Does not cross-react with other dopamine receptors.
This Anti-Dopamine D? Receptor Antibody is validated for use in ELISA, WB, Frozen Sections, Free-Floating Sections, Paraffin Sections for the detection of Dopamine D? Receptor.
免疫原
Human
a synthetic peptide (MDGTGLVVERDFSC) corresponding to amino acids 9-21 of human D?dopamine receptor
包装
100 μL in Plastic ampoule
应用
ELISA (see comments)
Immunoblotting (1:1000)
Frozen Sections (1:5000)
Free-Floating Sections (1:5000)
Paraffin Sections (not recommended)
警告
Toxicity: Standard Handling (A)
外形
Undiluted serum.
重悬
Reconstitute the antibody with 100 ?l PBS containing 1% BSA. For further dilution of the antibody use a similar buffer formulation. Following reconstitution, refrigerate (4°C).
分析说明
Positive Control
Rat brain
其他说明
Mignini, F., et al. 2000.J. Autonom. Pharmacol.20,325.
This antibody has also been reported to work in ELISA. For blocking immunoblot membranes, useONLY4% goat serum or evaporated goat′s milk. For frozen sections a 1:5000 dilution of the reconstituted serum demonstrates specific staining of rat brain sections including the medial septum, nucleus accumbens, dentate gyrus, globus pallidus, medial forebrain bundle, cortex regions 1-3, substantia nigra reticulata, and the ventral tegmental area. Strong nuclear staining of oligodendrocytes was also observed. Variables associated with assay conditions will dictate the proper working dilution.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
半岛bd体育手机客户端 性质
生物来源 | rabbit |
质量水平 | 100 |
抗体形式 | serum |
antibody product type | primary antibodies |
克隆 | polyclonal |
形式 | lyophilized |
不包含 | preservative |
species reactivity | mouse, rat, human |
manufacturer/tradename | Calbiochem? |
储存条件 | OK to freeze |
同位素/亚型 | IgG |
运输 | ambient |
储存温度 | 2-8℃ |
安全信息
储存分类代码 | 11 - Combustible Solids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich