Anti-phospho-ILK (Ser246) Antibody

半岛bd体育手机客户端 说明

一般描述

ILK (Integrin-Linked Kinase) is an intraceullar Ser/Thr kinase with four Ankyrin-like repeats. It is known to play pivotal roles in extracellular matrix mediated signaling. ILK is known to associate with β-Integrins in addition to many other focal adhesion associated proteins. In this context, ILK is involved cell adhesion and migration. The kinase activity appears to be reduced following integrin activation, and overexpression of p59 ILK inhibits cellular adhesion to integrin substrates. Additionally, ILK has been associated with epithelial mesechymal transition (EMT) in a TGF-β1 induced Smad dependent manner (Li, 2003). Forced expression of ILK also induced the expression of MMP-2 (matrix metalloprotease 2) and promoted cell migration and invasion (Li, 2003).

特异性

This antibody recognizes ILK when phosphorylated at Ser246.

免疫原

KLH-conjugated linear peptide corresponding to human ILK phosphorylated at Ser246.
Epitope: Phosphorylated Ser246

应用

Research Category
Cell Structure
Research Sub Category
Cytoskeletal Signaling
Detect phospho-ILK (Ser246) using this Anti-phospho-ILK (Ser246) Antibody validated for use in WB & IF.
Immunofluorescence Analysis: A previous lot was used by an independent laboratory in BT549 breast cancer cells. (Dr. Shoukat Dedhar, BC Cancer Research Centre, Vancouver, BC, Canada.)

质量

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.1 ?g/mL of this antibody detected ILK in 10 ?g of HeLa cell lysate.

目标描述

~54 kDa observed

外形

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
HeLa cell lysate

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	100
抗体形式	affinity isolated antibody
antibody product type	primary antibodies
克隆	polyclonal
纯化方式	affinity chromatography
species reactivity	rat, human, mouse
species reactivity (predicted by homology)	ox (based on 100% sequence homology)
technique(s)	immunofluorescence: suitable western blot: suitable
NCBI登记号	NP_004508
UniProt登记号	Q13418
运输	wet ice

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable