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位置: 首页> 品牌> Sigma-Aldrich> 抗三甲基组蛋白 H3 (Lys4) 抗体,克隆 15-10C-E4,兔单克隆

Anti-trimethyl-Histone H3 (Lys4) Antibody, clone 15-10C-E4, rabbit monoclonal

抗三甲基组蛋白 H3 (Lys4) 抗体,克隆 15-10C-E4,兔单克隆

品牌
Sigma-Aldrich
货号
05-745R
规格纯度
clone 15-10C-E4, Upstate?, from rabbit
价格
7365.52 *本价格含增值税费
促销
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数量
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半岛bd体育手机客户端 名称:
Anti-trimethyl-Histone H3 (Lys4) Antibody, clone 15-10C-E4, rabbit monoclonal
H3K4me3, Histone H3 (tri methyl K4)
抗三甲基组蛋白 H3 (Lys4) 抗体,克隆 15-10C-E4,兔单克隆
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Recombinant version of Cat. # 05-745, clone MC315

特异性

Broad species cross-reactivity expected due to sequence homology
Recognizes Histone H3 containing trimethyl-lysine 4 and, to a lesser extent, dimethyl-lysine 4. See certificate of analysis for further details.

免疫原

BSA-conjugated, synthetic peptide containing the sequence …RTme3KQT… in which me3K corresponds to trimethyl-lysine 4 of human histone H3.

应用

ChIP-seq Analysis:
Representative lot data. Chromatin immunoprecipitation was performed using the Magna ChIP HiSens kit (cat# 17-10460), 3 ?L of Anti-trimethyl-Histone H3 (Lys4) antibody (cat# 05-745R), 20 ?L Protein A/G beads, and 1e6 crosslinked HeLa cell chromatin followed by DNA purification using magnetic beads. Libraries were prepared from Input and ChIP DNA samples using standard protocols with Illumina barcoded adapters, and analyzed on Illumina HiSeq instrument. An excess of eighteen million reads from FastQ files were mapped using Bowtie (http://bowtie-bio.sourceforge.net/manual.shtml) following TagDust (http://genome.gsc.riken.jp/osc/english/dataresource/) tag removal. Peaks were identified using MACS (http://luelab.dfci.harvard.edu/MACS/), with peaks and reads visualized as a custom track in UCSC Genome Browser (http://genome.ucsc.edu) from BigWig and BED files. The highest 25% of peaks identified in the 05-745R and 07-473 datasets showed 99% overlap with peaks identified in the ENCODE H3K4me3 BROAD Histone track for HeLa S3.
Use Anti-trimethyl-Histone H3 (Lys4) Antibody, clone 15-10C-E4, Recombinant (rabbit monoclonal antibody) proven in WB, ChIP, ChIP-seq, ELISA, PIA, Mplex, DB to detect trimethyl-Histone H3 (Lys4) also known as H3K4me3, Histone H3 (tri methyl K4).
Research Sub Category
Histones
Research Category
Epigenetics & Nuclear Function

质量

Routinely evaluated by immunoblot on HeLa nuclear extract

目标描述

17 kDa

联系

Replaces: 05-745

外形

Format: Purified
Protein A purified, recombinant antibody in 60 mM Tris, 90 mM NaCl, 0.03% NaN3, 60 mM Glycine, pH 7.5, 40% Glycerol.

储存及稳定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.

法律信息

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss 32160702
NACRES NA.41

半岛bd体育手机客户端 性质

生物来源 rabbit
质量水平 100
抗体形式 purified antibody
antibody product type primary antibodies
克隆 15-10C-E4, monoclonal
species reactivity human
manufacturer/tradename Upstate?
technique(s) ChIP: suitable (ChIP-seq)
ELISA: suitable
dot blot: suitable
inhibition assay: suitable (peptide)
multiplexing: suitable
western blot: suitable
同位素/亚型 IgG
NCBI登记号 NM_003493
UniProt登记号 Q16695
运输 wet ice
Gene Information human ... H3F3B(3021)

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK WGK 1
闪点(F) Not applicable
闪点(C) Not applicable

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