半岛bd体育手机客户端 说明
一般描述
Recombinant version of Cat. # 05-745, clone MC315
特异性
Broad species cross-reactivity expected due to sequence homology
Recognizes Histone H3 containing trimethyl-lysine 4 and, to a lesser extent, dimethyl-lysine 4. See certificate of analysis for further details.
免疫原
BSA-conjugated, synthetic peptide containing the sequence …RTme3KQT… in which me3K corresponds to trimethyl-lysine 4 of human histone H3.
应用
ChIP-seq Analysis:
Representative lot data. Chromatin immunoprecipitation was performed using the Magna ChIP
Use Anti-trimethyl-Histone H3 (Lys4) Antibody, clone 15-10C-E4, Recombinant (rabbit monoclonal antibody) proven in WB, ChIP, ChIP-seq, ELISA, PIA, Mplex, DB to detect trimethyl-Histone H3 (Lys4) also known as H3K4me3, Histone H3 (tri methyl K4).
Research Sub Category
Histones
Research Category
Epigenetics & Nuclear Function
质量
Routinely evaluated by immunoblot on HeLa nuclear extract
目标描述
17 kDa
联系
Replaces: 05-745
外形
Format: Purified
Protein A purified, recombinant antibody in 60 mM Tris, 90 mM NaCl, 0.03% NaN3, 60 mM Glycine, pH 7.5, 40% Glycerol.
储存及稳定性
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
法律信息
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
半岛bd体育手机客户端 性质
生物来源 | rabbit |
质量水平 | 100 |
抗体形式 | purified antibody |
antibody product type | primary antibodies |
克隆 | 15-10C-E4, monoclonal |
species reactivity | human |
manufacturer/tradename | Upstate? |
technique(s) | ChIP: suitable (ChIP-seq) ELISA: suitable dot blot: suitable inhibition assay: suitable (peptide) multiplexing: suitable western blot: suitable |
同位素/亚型 | IgG |
NCBI登记号 | NM_003493 |
UniProt登记号 | Q16695 |
运输 | wet ice |
Gene Information | human ... H3F3B(3021) |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich