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位置: 首页> 品牌> Sigma-Aldrich> Anti-phospho-Akt (Thr308) Antibody, clone 50-1C-25, rabbit monoclonal

Anti-phospho-Akt (Thr308) Antibody, clone 50-1C-25, rabbit monoclonal

品牌
Sigma-Aldrich
货号
05-802R
规格纯度
clone NL50, Upstate?, from rabbit
价格
6526.19 *本价格含增值税费
促销
服务
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数量
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半岛bd体育手机客户端 名称:
Anti-phospho-Akt (Thr308) Antibody, clone 50-1C-25, rabbit monoclonal
Protein kinase B, RAC-alpha serine/threonine-protein kinase, murine thymoma viral (v-akt) oncogene homolog-1, rac protein kinase alpha, v-akt murine thymoma viral oncogene homolog 1
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Akt/PKB is a Ser/Thr kinase and a major known effecter of the PI3 Kinase pathway. It is involved in multiple signaling pathways that relate to many biological processes including metabolism, apoptosis, cell cycle control, angiogenesis, differentiation, and cell growth and proliferation. In mammals, three isoforms of Akt (Akt1/PKCα, Akt2/PKBβ, and Akt3/PKBγ) exists. They exhibit a high degree of homology, but differ slightly in the localization of their regulatory phosphorylation sites. Akt1 is the predominant isoform that is in most tissues and is thought to have a dominant role in growth, survival, embryonic development, and adipocyte differentiation. Akt2 is correlated with the regulation of glucose homeostasis and is the predominant isoform expressed in insulin-responsive tissues. Akt3 is abundant in brain tissue. Each Akt isoform is composed of three functionally distinct regions: an N-terminal Pleckstrin Homology (PH) domain that provides a lipid-binding module, a central catalytic domain containing Thr308, and a C-terminal hydrophobic motif containing Ser473. The activation of AKT is dependent on a dual regulatory mechanism that requires both its translocation to the plasma membrane and dual phosphorylation on Thr308 and Ser473 by PDK1 and the TORC2 complex, respectively.

特异性

Detects Akt only when phosphorylated on Thr308.

免疫原

KLH-conjugated synthetic peptide containing the Thr308 sequence and surrounding amino acids of human, mouse, rat, bovine and chicken Akt1.

应用

Research Category
Signaling
Detect phospho-Akt (Thr308) using this Anti-phospho-Akt (Thr308) Antibody, clone 50-1C-25 validated for use in WB, IF, IC, ELISA.
Research Sub Category
PI3K, Akt, & mTOR Signaling
Western Blot Analysis:
This lot detected Akt when phosphorylated on Thr308 at 1:1,000 dilution in PDGF-treated NIH/3T3 lysates via SDS-PAGE and transferred to PVDF (Immobilon-P).

质量

This lot detected Akt when phosphorylated on Thr308 at 1:1,000 dilution in PDGF-treated NIH/3T3 lysates via SDS-PAGE and transferred to PVDF (Immobilon-P).

目标描述

60 kDa

联系

Replaces: 05-802

外形

Format: Purified
Protein A purified
Protein A purified recombinant rabbit monoclonal supernatant in 0.1M Tris-Glycine (pH7.4) 150mM NaCl, and 0.05% NaN3 with 40% glycerol.

储存及稳定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

法律信息

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss 32160702
NACRES NA.41

半岛bd体育手机客户端 性质

生物来源 rabbit
质量水平 100
抗体形式 purified antibody
antibody product type primary antibodies
克隆 NL50, monoclonal
species reactivity human, mouse, rabbit, chicken, bovine
manufacturer/tradename Upstate?
technique(s) ELISA: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
western blot: suitable
同位素/亚型 IgG
NCBI登记号 NP_001014431
UniProt登记号 P31749
运输 wet ice

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK WGK 1
闪点(F) Not applicable
闪点(C) Not applicable

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