Anti-Ran antibody,Mouse monoclonal

半岛bd体育手机客户端 说明

一般描述

Monoclonal Anti-Ran (mouse IgG2b isotype) is derived from the ARAN1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BDF1 mice immunized with denatured recombinant human Ran. Ran is a small guanine trinucleotide phosphatase (GTPase) of the Ras superfamily that like other GTPases exist in two states: bound to guanine trinucleotide phosphate (GTP) or to guanine dinucleotide phosphate (GDP). The bound form is localized to the nucleus.

免疫原

denatured recombinant human Ran.

应用

Monoclonal Anti-Ran antibody produced in mouse has been used in:

• western blotting
• immunoprecipitation
• enzyme linked immunosorbent assay (ELISA)
• immunocytochemistry

生化/生理作用

Ran plays an important function in the directionality of nuclear transport. In this form it is important for the termination of the import reaction which is mediated by a complex of the cargo protein with importin-β and its adapter protein importin-α. The binding of RanGTP to importin-β causes the release of the cargo protein from the importin-α/importin-β complex. The import receptor (importin-β or transportin) is then exported out of the nucleus as a RanGTP complex and is released into the cytoplasm when RanGTP is converted to RanGDP by Ran-GAP1 (GTPase-activating protein). Export receptors respond in the opposite way by binding cargo with much higher affinity in the presence of RanGTP and exporting them out of the nucleus as RanGTP complexes. The exported cargo is released when Ran GAP1 converts RanGTP to RanGDP. In order to maintain the import/export cycle, Ran has to return to the nucleus. This is achieved by a small protein called nuclear transport factor 2 (NTF2) also known as p10, that binds RanGDP in the cytoplasm, and delivers it back into the nucleus. In the nucleus RanGDP encounters RanGEF (guanine nucleotide exchange factor) and undergoes nucleotide exchange to become RanGTP.

外形

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号	MFCD03792430
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	200
生物来源	mouse
偶联物	unconjugated
抗体形式	purified from hybridoma cell culture
antibody product type	primary antibodies
克隆	ARAN1, monoclonal
形式	buffered aqueous solution
species reactivity	bovine, human,Xenopus, mouse, hamster
technique(s)	immunocytochemistry: suitable immunoprecipitation (IP): suitable indirect ELISA: suitable microarray: suitable western blot: 0.5-1 μg/mL using total extract of Jurkat cells
同位素/亚型	IgG2b
UniProt登记号	P62826
运输	dry ice
储存温度	?20℃
Gene Information	human ... RAN(5901) mouse ... Ran(19384)