半岛bd体育手机客户端 说明

一般描述

This antibody reacts specifically with non-reduced rabbit IgG and does not react with reduced rabbit IgG.
Anti-Rabbit IgG, Native antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma RabT-50 produced by the fusion of mouse myeloma cells (NS1) and splenocytes from BALB/c mice. Immunoglobulin G (IgG) is found abundantly in the human serum. It constitutes about 10-20% of the plasma proteins. IgG is composed of glycoproteins, out of which it is 82-96% proteins and 4-18% carbohydrates. It consists of four sub-classes i.e IgG1, IgG2, IgG3, and IgG4. IgG is composed of four polypeptide chains-two heavy chains (γ?chains) and two light chains (κ or λ chains) which are linked by inter-chain disulfide bonds. The heavy and the light chains are linked at VH and CH1 domain to form the Fragment antigen binding (Fab) arm. The antigen binds to the V regions of the antibody.

特异性

Anti-Rabbit IgG, Native antibody, Mouse monoclonal reacts specifically with non-reduced rabbit IgG and does not react with reduced rabbit IgG. It does not recognize human, monkey, bovine, horse, pig, goat, cat, rat, and chicken IgGs.

免疫原

Enriched fraction of rabbit IgG light chain.

应用

Anti-Rabbit IgG, Native antibody, Mouse monoclonal has been used in:

• immunoblotting
• immunocytochemistry
• immunohistochemistry
• enzyme-linked immunosorbent assay (ELISA)

生化/生理作用

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.

外形

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

储存及稳定性

For extended storage, freeze at –20 ℃ in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discard if not used within 12 hours.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

NACRES

NA.46

半岛bd体育手机客户端 性质

质量水平	200
生物来源	mouse
偶联物	unconjugated
抗体形式	purified immunoglobulin
antibody product type	secondary antibodies
克隆	RabT-50, monoclonal
形式	buffered aqueous solution
species reactivity	rabbit
should not react with	horse, monkey, cat, human, goat, pig, bovine, rat, chicken
浓度	~1 mg/mL
technique(s)	immunocytochemistry: suitable indirect ELISA: suitable western blot: 1-2 μg/mL using 10 ng of non-reduced rabbit IgG (Cat. No. I5006) per lane
同位素/亚型	IgG1
运输	dry ice
储存温度	?20℃

安全信息

储存分类代码	12 - Non Combustible Liquids
闪点(F)	Not applicable
闪点(C)	Not applicable
个人防护装备	Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter