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Ribonucleic acid, transfer from bovine liver

品牌
Sigma-Aldrich
CAS
9014-25-9
货号
R4752
规格纯度
Type XI, lyophilized powder
价格
5799.16 *本价格含增值税费
促销
服务
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  • 包邮
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数量
- +
半岛bd体育手机客户端 名称:
9014-25-9
Ribonucleic acid, transfer from bovine liver
Transfer RNA
tRNA
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Transfer ribonucleic acid (tRNA) is a non-coding RNA. It is approximately 70-100 base pair long. It has a cloverleaf shaped secondary structure. It has three or four arms and a stem structure. It has minor bases like pseudouridine, dihydrouridine and methylated bases in its structure. It has a binding site for triplet nucleotide sequence and for amino acids.

应用

Ribonucleic acid, transfer from bovine liver has been used:

  • to analyze the efficiency of thioflavin T (ThT) as a fluorophore
  • to test its ability to inhibit the growth of endothelial cells
  • as a component in translation mixture
  • as a non-specific competitor RNA for protein purification studies using poly(A) tailed RNA affinity column


Ribonucleic acid, transfer from bovine liver may be used as a starting material for the purification of specific amino acyl-tRNA species by methods such as liquid column chromatography or high-performance liquid chromatography (HPLC).

包装

50, 100 units in serum bottle

生化/生理作用

Transfer ribonucleic acid (tRNA) plays a major role in translation, a key step in protein synthesis. It decodes mRNA into protein. There are about 40-60 different tRNAs in a eukaryotic cell and fewer in bacteria. This depends on the degeneracy of the amino acid code. Each amino acid has a specific tRNA. tRNAs are charged with specific amino acid and carries them to the site of protein synthesis. It binds to the ribosome and directs the amino acid to the growing polypeptide chain. It also functions as a regulator of various biological processes. Mutations associated with tRNA is implicated in human diseases.

单位定义

一单位在 1.0mL 水(1cm 光程)中的 A260为 1.0。

分析说明

This product is evaluated for amino acceptor activity using aminoacyl-tRNA synthetase and L-arginine

基本信息

MDL编号 MFCD00132200

半岛bd体育手机客户端 性质

质量水平 200
类型 Type XI
形式 lyophilized powder
储存温度 ?20℃

安全信息

储存分类代码 13 - Non Combustible Solids
WGK WGK 3
闪点(F) Not applicable
闪点(C) Not applicable
个人防护装备 Eyeshields, Gloves, type N95 (US)

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